中国比较医学杂志2017，Vol.27Issue(7)：6-12,7.DOI:10.3969.j.issn.1671-7856.2017.07.002
幽门螺杆菌感染小鼠慢性胃炎模型的建立及评价
Establishment and evaluation of a mouse model of chronic gastritis induced by Helicobacter pylori
摘要
Abstract
Objective To establish a mouse model of H.pylori infection, and to evaluate the chronic pathological changes in the gastric mucosa associated with H.pylori infection.Methods 34 male 5~6-week old SPF C57BL/6 mice were used in this study.The mice were intragastrically administrated with a suspension of H.pylori SS1 strain.Two weeks after infection, rapid urease test and PCR were performed to confirm the H.pylori infection.Successfully infected mice were randomly divided into 3 groups including the control group, 6-week and 12-week infected groups.Samples of gastric mucosa were taken for pathological analysis using HE and borax methylene blue staining.The contents of myeloperoxidase (MPO), superoxide dismutase (SOD), malondialdehyde (MDA) and catalase (CAT) in the gastric tissues were detected by biochemistry, and the expression levels of COX-2, iNOS, TNF-α and IL-1β were examined by RT-qPCR.Results Compared with the control group, H.pylori colonization was observed in the gastric mucosa of the 6-week and 12-week infected mice, with chronic inflammatory cell infiltration, glandular atrophy and intestinal metaplasia to varying extents.The contents of CAT and SOD were significantly decreased, while the levels of MPO and malonaldehyde MDA, and the expression levels of COX-2,iNOS,TNF-α and IL-1β were significantly increased (P<0.05 or P<0.01).Conclusions Intragastric administration with H.pylori in C57BL/6 mice can be successfully used to generate the bacterial colonization, leading to chronic inflammatory cell infiltration, enhanced oxidative stress, and up-regulated expression of proinflammatory genes in the gastric glandular tissues at 6 and 12 weeks after inoculation.However, the inflammatory changes are more extensive in the mice at 12 weeks after infection, with glandular atrophy and intestinal metaplasia.关键词
幽门螺杆菌/慢性胃炎/小鼠模型/氧化应激/促炎因子Key words
Helicobacter pylori/Chronic gastritis/Mouse model/Oxidative stress/Proinflammatory factors分类
医药卫生引用本文复制引用
连大卫,扶丽君,许艺飞,任文康,操红缨,黄萍..幽门螺杆菌感染小鼠慢性胃炎模型的建立及评价[J].中国比较医学杂志,2017,27(7):6-12,7.基金项目
国家自然科学基金资助项目(编号:81374043) （编号:81374043）
广东省科技计划资助项目(编号:2016A020217019) （编号:2016A020217019）
广州市科技计划资助项目(编号:201607010336) （编号:201607010336）
广东省省级科技计划项目(编号:2013A022100001). （编号:2013A022100001）
