中国比较医学杂志2017，Vol.27Issue(7)：13-16,33,5.DOI:10.3969.j.issn.1671-7856.2017.07.003
富氢液通过PI3K通路减轻大鼠脑缺血再灌注损伤
Hydrogen-rich saline reduces cerebral ischemia-reperfusion injury in rats via PI3K pathway
摘要
Abstract
Objective To investigate the effect of hydrogen-rich saline on apoptosis in hippocampal neurons induced by cerebral ischemia-reperfusion in rats and PI3K/Akt/FoxO1 signaling pathway.Methods The rat model of focal cerebral ischemia-reperfusion was established by thread-occlusion of the middle cerebral artery in rats.SD rats were randomly divided into sham operation group (Sham group), ischemia-reperfusion group (I/R group) and hydrogen-rich saline treatment group (HRS group), 10 rats in each group.At 24 h after reperfusion, the serum levels of IL-6, TNF-a and IL-1β were detected by ELISA.Histological changes of the hippocampus were observed by pathology using HE staining.Apoptosis in brain tissues was observed by TUNEL staining.The expression changes of p-PI3K, Akt, caspase-3 and FoxO1 proteins were detected by Western blot assay.Results Compared with the sham group, pyramidal cells were arranged loosely in the I/R group and a large number of pyramidal cells were necrotized, and the amount of apoptotic hippocampal cells was increased.The levels of IL-1β, IL-6 and TNF-α were significantly increased (P< 0.05), as well as the expression of p-PI3K, Akt and caspase-3 in the brain tissue.However, the expression of FoxO1 protein was decreased.There were significant differences between the two groups (P< 0.05).Compared with the I/R group, the inflammatory factors were significantly decreased in the HRS group.The expressions of p-PI3K, Akt and caspase-3 were also significantly decreased, while the expression of FoxO1 protein was increased (P< 0.05).Conclusions Hydrogen-rich saline can reduce brain injury caused by ischemia-reperfusion, and its mechanism may be related to PI3K/Akt/FoxO1 signaling pathway.关键词
富氢液/缺血再灌注/PI3K/FoxO1/细胞凋亡Key words
Hydrogen rich saline/Ischemia reperfusion/PI3k/FoxO1/Apoptosis分类
医药卫生引用本文复制引用
陈克研,董婉维..富氢液通过PI3K通路减轻大鼠脑缺血再灌注损伤[J].中国比较医学杂志,2017,27(7):13-16,33,5.基金项目
国家自然青年科学基金项目(31201758) （31201758）
中国医科大学新教师基金项目(XZR20160036). （XZR20160036）
