中国比较医学杂志2017，Vol.27Issue(7)：24-28,5.DOI:10.3969.j.issn.1671-7856.2017.07.005
小鼠间断性禁食模型的建立及其对葡萄糖内稳态的影响
Establishment of a mouse model of intermittent fasting and characterization of its effect on glucose homeostasis
摘要
Abstract
Objective To establish a stable and reliable mouse model as an alternative to the traditional model of impaired glucose tolerance induced by calorie restriction and its effect on glucose homeostasis.Methods Forty 16-week-old SPF C57BL/6J mice (half male and half female) were randomly divided into four groups by sex and the way of feeding.The mice in the ad libitum (AL) group had free access to basic diet, while the mice in the intermittent fasting (IF) group had normal diet and fasting on alternate days, with free access to water on the fasting days.The changes of body weight and blood glucose concentration in each group were monitored, and intraperitoneal glucose tolerance test and insulin tolerance test in mice were performed before and after the 12-week IF treatment.Results At 12 weeks after IF treatment, the body weight and blood glucose concentration of mice did not show significant difference.After i.p.injection of glucose, the blood glucose concentration of IF mice was less increased than the AL group, and after the insulin injection, the blood glucose concentration was more decreased.Compared to the AL group, the areas under the curve of tolerance test in the IF group were significantly decreased (P < 0.05).Conclusions After IF treatment, the mice show an enhanced sensitivity to insulin and improved glucose tolerance.This establishment method of mouse model of intermittent fasting is easy and simple, therefore, can be used as an effective alternative to traditional calorie restriction model of impaired glucose tolerance.关键词
热量限制/间断性禁食/葡萄糖内稳态/胰岛素Key words
Caloric restriction/Intermittent fasting/Glucose homeostasis/Insulin分类
医药卫生引用本文复制引用
马澜婧,张浩浩,丁美玲,张成博,倪耀宗,田祖宏,唐光波,窦建华,聂勇战..小鼠间断性禁食模型的建立及其对葡萄糖内稳态的影响[J].中国比较医学杂志,2017,27(7):24-28,5.基金项目
国家自然科学基金重大国际合作项目(编号:81120108005). （编号:81120108005）
