中国比较医学杂志2017，Vol.27Issue(7)：40-47,8.DOI:10.3969.j.issn.1671-7856.2017.07.008
高通量测序法研究不同pH的饮用水对SPF小鼠肠道微生物的影响
Effect of drinking water at different pH on intestinal microbial diversity in SPF mice evaluated by high throughput sequencing
摘要
Abstract
Objective To study the effect of drinking water at different pH on intestinal microbial diversity in SPF mice.Methods Ninety 21-day-old SPF mice were randomly divided into three groups: drinking acidified water (pH 3.0), neutral water (pH 7.0) and alkaline water (pH 9.0), respectively, for 3 months, 30 mice in each group.10 mice were sacrificed at the end of 4, 8 and 12 weeks in each group.Bacteria, virus and parasites in serum were detected according to the national standard for SPF.Then, primer sequences were designed according to the conservativeness of bacteria 16S rRNA, and libraries were constructed.Subsequently, the ileocecal contents were subjected to high-throughput sequencing (Illumina).10 G data were obtained and analyzed.Results The sequencing results revealed that the intestinal microbial abundance of the mice receiving pH 3.0 drinking water was moderate with a high stability.Conclusions Under the conditions of large-scale breeding, the mice receiving pH 3.0 drinking water can maintain the intestinal microbial diversity for a long time, and reach a stable platform.This study provides theoretical and technical support for the stable long-term maintenance of animal quality production.It is also a specific application of gastrointestinal macrogenomics in animal quality control.关键词
肠道微生物/16SrRNA/高通量测序/饮用水/小鼠Key words
Intestinal microflora/16S rRNA/High throughput sequencing/Drinking water/Mice分类
医药卫生引用本文复制引用
余强,刘月环,王志远,吴旧生..高通量测序法研究不同pH的饮用水对SPF小鼠肠道微生物的影响[J].中国比较医学杂志,2017,27(7):40-47,8.基金项目
浙江省公益技术应用研究计划(2011C37096,2015C37102) （2011C37096,2015C37102）
浙江省医药卫生平台骨干人才基金(2015RCA006) （2015RCA006）
浙江省自然科学基金(LY16H030011). （LY16H030011）
