中国比较医学杂志2017，Vol.27Issue(7)：87-92,6.DOI:10.3969.j.issn.1671-7856.2017.07.016
微卫星技术在NIH小鼠群体遗传结构分析中的应用
Application of microsatellite technology in the genetic structure analysis of NIH mice
郭羽 1王洪 2魏杰 2王锡岩 1李晓晖 1闵凡贵 3岳秉飞2
作者信息
- 1. 北京天坛生物制品股份有限公司,北京 100176
- 2. 中国食品药品检定研究院,北京 100050
- 3. 广东省实验动物监测所,广东省实验动物重点实验室,广州 510663
- 折叠
摘要
Abstract
Objective To compare and analyze the genetic structure of NIH mice bred in Unites A and B, using microsatellite technology.Methods Thirty SPF 8-week old outbred NIH mice (half male and half female) of each population were randomly chosen from the Units A and B, respectively.PCR amplification and STR scan were performed to determine the genetic characteristics of two outbred populations using microsatellite loci, and the population genetic structure was analyzed with statistical software Popgene 1.32.Results In the NIH mouse population form the Unit A, 74 alleles were obtained, with an average heterozygosity of 0.3108 and polymorphism information content of 0.2637.In the NIH mouse population from the Unit B, 76 alleles were obtained, with an average heterozygosity of 0.3257 and polymorphism information content of 0.2777.The inter-population comparison showed that genetic differentiation coefficient Fst was 0.3932, the genetic identity was 0.3971, and the genetic distance was 0.9235.The population difference was significant.Conclusions There is serious genetic differentiation between the two NIH mice populations,resulting in the formation of two different closed populations.关键词
封闭群/NIH小鼠/微卫星DNA/遗传结构Key words
Outbred population/NIH mice/Microsatellites DNA/Genetic structure分类
医药卫生引用本文复制引用
郭羽,王洪,魏杰,王锡岩,李晓晖,闵凡贵,岳秉飞..微卫星技术在NIH小鼠群体遗传结构分析中的应用[J].中国比较医学杂志,2017,27(7):87-92,6.
