中国实用口腔科杂志2017，Vol.10Issue(6)：354-359,6.DOI:10.19538/j.kq.2017.06.008
不同铜含量的钛铜合金对成骨细胞黏附与迁移的影响研究
Effects of different copper content of titanium-copper alloy on adhesion and migration of osteoblasts
摘要
Abstract
Objective To study the effect of different copper content of titanium-copper alloy on adhesion and migration of osteoblasts,and to find the optimal copper content.Methods The titanium-copper alloy with copper content of 2％,5％ and 10％ was prepared and named Ti-2wt％ Cu,Ti-5wt％ Cu and Ti-10wt％ Cu,respectively.The pure titanium material without copper was used as the control,Ti group.Mouse MC3T3-E1 osteoblasts were cultured on the surface of these materials.(1)Morphological observation of osteoblasts:the surface morphology of osteoblasts was observed;the cytoskeleton-actin staining was observed.(2)The proliferation of osteoblasts was detected by MTT assay.(3) Cell migration was analyzed by cell scratch test.SPSS 17.0 software was used for statistical analysis.Results (1)Under scanning electron microscope:cells showed more rapid and fuller adhesion and expansion on the Ti-5wt％Cu,Ti-10wt％Cu when the cells were seeded for 6 and 24 hours,but Ti-5wt％ Cu was more obvious.(2)By phalloidine staining:the cytoskeleton actin filaments were observed only in the Ti-10wt％Cu group when the cells were inoculated with phalloidin for 2h.The spreading area of Ti-5wt％Cu was the largest at 4h and 24h.(3)The MTT results showed:the number of osteoblasts on the surface of materials increased with the increase of copper content,that is,Ti-10wt％ Cu ＞ Ti-5wt％ Cu ＞ Ti-2wt％ Cu ＞ Ti,the difference being statistically significant (P ＜ 0.05).However,there was no significant difference between Ti-1Owt％ Cu and Ti-5wt％ Cu on the 7th day.(4)After 24 and 48 hours of scratches,the average width of scratches was Ti ＞ Ti-2wt％ Cu ＞ Ti-1Owt％ Cu＞Ti-5wt％ Cu and there was significant difference among the four groups (P ＜ 0.05).Conclusion In the range of copper content detected in this experiment,osteoblast adhesion,the formation of cytoskeleton and cell migration are the best in the range of 5％ of copper content.关键词
钛铜合金/成骨细胞/细胞形态/细胞增殖/细胞迁移Key words
titanium-copper alloy/osteoblast/cell morphology/cell proliferation/cell migration分类
医药卫生引用本文复制引用
李悦,张燕婷,何晶,马凯,王敏雅,李胜义,邓春富,张二林,赵宝红..不同铜含量的钛铜合金对成骨细胞黏附与迁移的影响研究[J].中国实用口腔科杂志,2017,10(6):354-359,6.基金项目
沈阳市科技计划项目(F11-264-1-25) （F11-264-1-25）
辽宁省科技计划项目(2013225049) （2013225049）
辽宁省自然科学基金(20170541059) （20170541059）
