中国实用口腔科杂志2017，Vol.10Issue(6)：373-377,5.DOI:10.19538/j.kq.2017.06.
has-miR-16在舌鳞癌中的生物学功能及分子机制研究
Biological function and molecular mechanism of has-miR-16 in tongue squamous cell carcinoma
摘要
Abstract
Objective To study the role and molecular mechanism of has-miR-16 in the development of tongue squa mous cell carcinoma (TSCC).Methods The expression of has-miR-16 was detected in 36 cases of TSCC tissues and matched normal tissues by using Quantitative real time PCR.The expression of has-miR-16 in TSCC cells (CAL-27 and SCC-9) and the normal human oral mucosa fibroblast cells (hOMF) was also analyzed by Quantitative real time PCR.The cell proliferation and migration of CAL-27 and SCC-9 were analyzed by MTT and wound healing assays after being transfected with has-miR-16 mimics or mimics control.The target gene of has-miR-16 was predicted by bioinformatics and verified by dual luciferase reporter assay.Results The expression of has-miR-16 in TSCC tissues was significantly lower than matched normal tissues (P ＜ 0.05).The expression of has-miR-16 was also down-regulated in CAL-27 and SCC-9 cells compared with hOMF cells (P ＜ 0.05).Over-expression of has-miR-16 inhibited cell proliferation and migration in CAL-27 and SCC-9 cells (P ＜ 0.05).MYB (Myeloblastosis oncogene) was the target gene of has-miR-16 in TSCC.Conclusion Has-miR-16 is down-regulated in TSCC tissue and cell lines;over-expression of has-miR-16 inhibites CAL-27 and SCC-9 cells proliferation and migration;has-miR-16 acts as tumor suppressor in TSCC,which may play its role via regulating MYB.关键词
has-miR-16/舌鳞癌/抑癌作用/成髓细胞瘤癌基因Key words
has-miR-16/tongue squamous cell carcinoma, TSCC/suppressor/myeloblastosis oncogene, MYB分类
医药卫生引用本文复制引用
李琦,耿亚军,郭菁,鄂丽华,徐溢涵,金武龙..has-miR-16在舌鳞癌中的生物学功能及分子机制研究[J].中国实用口腔科杂志,2017,10(6):373-377,5.基金项目
内蒙古医科大学科技百万工程项目(2014YKDKJBW01) （2014YKDKJBW01）
内蒙古医科大学人才团队项目:口腔再生医学团队(NYTD-2015011) （NYTD-2015011）
