中国现代手术学杂志2017，Vol.21Issue(2)：85-89,5.DOI:10.16260/j.cnki.1009-2188.2017.02.002
肿瘤相关巨噬细胞和细胞因子表达对三阴乳腺癌患者预后的影响
The Expression of Tumor Associated Macrophages and Cytokines in the Triple-negative Breast Cancer Patients after the Surgery
王建国 1李绍杰1
作者信息
- 1. 湖南省湘潭市中心医院普外科,湘潭411100
- 折叠
摘要
Abstract
Objective To explore the expressional value of tumor associated macrophages (TAMs) and cytokines in the prognosis of triple-negative breast cancer (TNBC).Methods Immunohistochemistry (IHC) and S-P staining method were used to detect the TAMs marker CD68 expression in 48 TNBC samples.Expressions of key cytokines including interleukin-6 (IL-6),IL-10,IL-12,IL-1 β,chemokine (C-C motif) ligand-5 (CCL-5) and macrophage inflammatory protein-2 (MIP-2) were quantified by RT-PCR and enzyme-linked immunosorbent assay (ELISA).The correlation between CD68 expression and prognosis was analyzed.Results 34 cases(70.8％) out of 48 TNBC samples appeared CD68-positive expression.Compared to low infiltrated samples,IL-6 and CCL-5 were up-regulated in the high infiltrated tumors samples (P ＜0.01),but there was no statistical difference in other cytokines between low and high infihrated tumor samples.The survival rate of high infiltrated tumor samples were lower than that of low infiltrated tumor sample (P ＜ 0.05).Conclusions Theexpression of TAMs were up-regulated in most of TNBC patients after the surgery.Its expression suggested unfavorable prognosis especially in high-infiltrated group.Those tumors with more macrophage also had elevated expression of cytokine(IL-6) and chemotactic factor (CCL-5),both of which have potency to be clinical index and drug target for TNBC.关键词
乳腺癌,三阴性/肿瘤相关巨噬细胞/白细胞介素6/ccl-5Key words
breast cancer, triple-negative/tumor-associated macrophages/interleukin-6/ccl-5分类
医药卫生引用本文复制引用
王建国,李绍杰..肿瘤相关巨噬细胞和细胞因子表达对三阴乳腺癌患者预后的影响[J].中国现代手术学杂志,2017,21(2):85-89,5.
