中国现代医学杂志2017，Vol.27Issue(14)：1-7,7.DOI:10.3969/j.issn.1005-8982.2017.14.001
姜黄素衍生物对脂多糖诱导急性肺损伤的防护作用研究
Curcumin derivative protects mouse from acute lung injury induced by lipopolysaccharide
摘要
Abstract
Objective To investigate the protective and therapeutic effect of curcumin derivative on lipopolysaccharide-stimulated acute lung injury (ALI) in mice so as to provide reference for the study on mechanism and clinic application of curcumin derivative. Methods The mouse model of ALI was built by intra-peritoneal injection of lipopolysaccharide (LPS). Ninety Kunming mice were randomly divided into 6 groups (each with 15): normal control, ALI, curcumin (100 mg/kg), curcumin derivative (50, 100 and 200 mg/kg) groups. The mice were pretreated with gavage of different medicines for 7 days, and had intraperitoneal injection of LPS (10 mg/kg) on the 7th d. The changes of proportions of neutrophils and monocytes in blood were detected 6 h, 1 and 3 d after establishment of the model, meanwhile, the content of malondialdehyde (MDA), myelo-peroxidase (MPO) and glutathione (GSH) in the lung tissues was tested and histological changes of the lungs were observed. The expressions of TNF-α and IL-6 in the lung tissues were measured by ELISA. Results The curcumin derivative reduced the number of inflammatory cells in blood and lowered the production of inflam-matory factors. Histological studies showed that there were congestion, edema and infiltration of inflammatory cells in the lung tissues of the ALI group. Compared with the ALI group, the histological changes were im-proved greatly in the curcumin derivative groups ( <0.05). Conclusions Curcumin derivative can protect mice from ALI induced by LPS, the mechanism will be studied in the next experiment.关键词
急性肺损伤/姜黄素衍生物/脂多糖/肿瘤坏死因子-α/白介素6Key words
acute lung injury/curcumin derivative/lipopolysaccharide/tumor necrosis factor α/inter-leukin-6分类
医药卫生引用本文复制引用
王娟娟,刘斌,曹波,魏路清..姜黄素衍生物对脂多糖诱导急性肺损伤的防护作用研究[J].中国现代医学杂志,2017,27(14):1-7,7.基金项目
国家自然科学基金青年科学基金(No:81600051) （No:81600051）
国家自然科学基金重点项目(No:71533008) （No:71533008）
