中国现代医学杂志2017，Vol.27Issue(14)：14-18,5.DOI:10.3969/j.issn.1005-8982.2017.14.003
热射病大鼠死亡危险因素及心肌损伤的实验研究
Death risk factors and myocardial injury in rat model of heat stroke
摘要
Abstract
Objective To establish the rat model of heat stroke, explore the risk factors of death and myocardium injury in the rat model. Methods Forty-eight male SD rats of SPF grade were randomly divided into group of blank control (C), group of heat stroke (HS), group of heat stroke vitamin E treatment (VitE) and group of heat stroke Valsartan treatment (Valsartan), each group included 12 rats. Except the group C, the rats of other groups were put in a damp and hot environment of 40℃ (temperature) and (65 ± 1) %(humidity) until the standard of the model of heat stroke was reached. The core temperature of the bodies of rats were monitored via the record of rectal temperature. The responses of heat stress in the HS, VitE HS and Valsartan HS groups and the survival time of rats were analyzed and compared by K-M survival analysis. The risk factors of survival were analyzed by univariate and multivariate Cox regression analyses. The lesions of myocardium were observed through HE staining and transmission electron microscopy (TEM). Results The rate of weight change in the group C was significantly lower than that in the groups HS, VitE HS and Valsartan HS ( <0.05). The serum level of acetylcholine (Ach) in the group C [(15.734 ± 4.987)μg/ml] was significantly higher in that in the group HS [(9.958 ± 4.283) μg/ml, <0.05)]. The results of HE staining and TEM showed that there was myocardium injury in the rats with heat stroke, which was milder in the groups VitE HS and Valsartan HS compared with the group HS. Conclusions Death mainly occurs in 24 hours after the onset and the major risk factors are low body temperature and long time of striking, but the relatively high rate of weight change is the protective factor of the rat survival. Myocardium injury exists in the rat model of heat stroke, the potential mechanism may be apoptosis of myocardial cells induced by oxidative stress and angiotensin Ⅱ.关键词
热射病/动物模型/心肌损伤/氧化应激/死亡率Key words
heat stroke/animal model/myocardial injury/oxidative stress/mortality分类
医药卫生引用本文复制引用
陈长富,袁彬彬,董文鹏,刘莹,王晓武..热射病大鼠死亡危险因素及心肌损伤的实验研究[J].中国现代医学杂志,2017,27(14):14-18,5.基金项目
国家自然科学基金(No:81671885) （No:81671885）
广东省自然科学基金(No:2015A030313612) （No:2015A030313612）
广东省科技计划(No:2014A020212262) （No:2014A020212262）
