中国现代医学杂志2017，Vol.27Issue(14)：19-23,5.DOI:10.3969/j.issn.1005-8982.2017.14.004
-2基因转染骨髓干细胞治疗激素性股骨头坏死的疗效研究
Treatment of steroid-induced femoral head necrosis with Bcl-2 gene modified bone marrow mesenchymal stem cells
摘要
Abstract
Objective To explore the curative effect of implantation of bone marrow mesenchymal stem cells (BMSCs) transfected with Bcl-2 combined with core decompression on experimental femoral head necrosis in rabbits. Methods Forty rabbits were selected, and femoral head necrosis models were successfully prepared in 28 rabbits. Then, the model rabbits were randomized into 4 groups: blank control group ( =6) without treat-ment, core decompression group ( =6), BMSCs group ( =8) subjected to core decompression and implantation of BMSCs, and Bcl-2 group ( =8) subjected to core decompression and implantation of Bcl-2-transfected BMSCs. In the 8th and 12th week after implantation, femoral head tissues were taken and sliced into sections for pathological examination and detection of osteocyte apoptosis. Results gene was successfully trans-fected into BMSCs. At the 8th week after implantation, there was only a little new bone formation in the core decompression group and the BMSCs group; at the 12th week, fibrous tissues were formed in the decompression channel. In the Bcl-2 group, new bone formation was observed, and at the 12th weeks, the necrotic region was completely repaired. The positive TUNEL staining of the Bcl-2 group was much less than that of the other groups, the differences were significant ( <0.05). Conclusions These findings indicate that Bcl-2-transfected bone marrow mesenchymal stem cell transplantation via core decompression has better curative effect on exper-imental femoral head necrosis in rabbit model.关键词
骨髓间充质干细胞/-2基因/股骨头坏死/髓芯减压Key words
bone marrow mesenchymal stem cell/Bcl-2 gene/femoral head necrosis/core decompression分类
医药卫生引用本文复制引用
张立岩,王海涛,孙新,田丹,罗勇,陈继营,柴伟,王华,徐睿,刘鑫..-2基因转染骨髓干细胞治疗激素性股骨头坏死的疗效研究[J].中国现代医学杂志,2017,27(14):19-23,5.基金项目
吉林省科技发展计划(No:20150312013ZG) （No:20150312013ZG）
吉林省教育厅"十二五"科学技术研究项目[No:吉教科合字(2015)第141号] （2015）
吉林省教育厅"十三五"科学技术研究项目[No:吉教科合字(2016)第63号] （2016）
