中国现代医学杂志2017，Vol.27Issue(14)：28-32,5.DOI:10.3969/j.issn.1005-8982.2017.14.006
溪黄草黄酮对小鼠非酒精性脂肪肝纤维化的影响及其机制探讨
Effect of flavonoids from ( Maxim) on fibrosing nonalcoholic steatohepatitis in mice
摘要
Abstract
Objective To investigate the effect of flavonoids from a( Maxim) (FRSH) on fibrosing nonalcoholic steatohepatitis (NASH) in mice and discuss the mechanism. Methods Mice were randomly divided into five groups: control group, model group, and treatment groups (FRSH-L, FRSH-M and FRSH-H groups). The mice in the model group were given high-fat and methionine-choline deficient (MCD) diet. In the treatment groups MCD diet and different concentrations of FRSH were used. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were examined. The pathological changes of the liver were observed after HE staining. The expressions of MMP-9 and TIMP-1 in the liver were determined by Western blot. The mRNA levels of TGF-β and PPARγ were detected by qRT-PCR. Results Compared with the model group, treatment with FRSH significantly decreased the serum levels of ALT and AST and improved the liver inflammation and fibrosis. Moreover, the expressions of TIMP-1 protein and TGF-β mRNA were reduced and the expressions of MMP-9 protein and PPARγ mRNA were increased in the liver after FRSH treatment. Conclusions FRSH could retard the development of fibrosing nonalcoholic steatohepatitis through modulation of the expressions of MMP-9, TIMP-1, TGF-β and PPARγ.关键词
溪黄草黄酮/非酒精性脂肪性肝纤维化/基质金属蛋白酶-9/基质金属蛋白酶抑制剂-1/转化生长因子-β/过氧化物酶体增殖物活化受体-γKey words
flavonoids from Rabdosia serra (Maxim.) hara/fibrosing nonalcoholic steatohepatitis/MMP-9/TIMP-1/TGF-β/PPARγ分类
医药卫生引用本文复制引用
郑玉峰,张英剑,郭虹,高磊,苏秀丽..溪黄草黄酮对小鼠非酒精性脂肪肝纤维化的影响及其机制探讨[J].中国现代医学杂志,2017,27(14):28-32,5.基金项目
河南省医学科技公关计划(No:201504039) （No:201504039）
