中国现代医学杂志2017，Vol.27Issue(14)：33-39,7.DOI:10.3969/j.issn.1005-8982.2017.14.007
lncRNA-ATB促进结肠癌细胞侵袭与转移的机制研究
LncRNA-ATB promotes invasion and metastasis of colon cancer cells
李薇 1孙锋 2吴雪松 2唐浩然 2何水旺3
作者信息
- 1. 昆明医科大学第二附属医院 检验科,云南 昆明 650101
- 2. 昆明医科大学第二附属医院胃肠外科一病区,云南 昆明 650101
- 3. 中国科学院昆明动物研究所 分子与病理实验室,云南 昆明 650223
- 折叠
摘要
Abstract
Objective To investigate the effect of long non-coding RNA activated by TGF-β (LncRNA-ATB) on the migration and invasion of human colorectal cancer cells and the underlying mechanism. Methods The efficiency of LncRNA-ATB shRNA and pcDNA3.1-LncRNA-ATB, and the expressions of LncRNA-ATB and IL-11 mRNA were detected by quantitative real time PCR (qRT-PCR). The effect of LncRNA-ATB on migration and invasion abilities of colorectal cells were analyzed by cell migration and cell invasion assays. The binding ability of LncRNA-ATB and IL-11 mRNA was analyzed by RNA immunoprecipitation (RIP). The expression of IL-11 protein was analyzed by ELISA. Results The expression of LncRNA-ATB in the colorectal cells was higher than that in the normal human colon epithelial cells. LncRNA-ATB expression in the HT29 cells could be effectively silenced by LncRNA-ATB shRNA and LncRNA-ATB expression in the SW620 cells could be efficiently upregulated by pcDNA3.1-LncRNA-ATB. LncRNA-ATB could significantly promote the migration and invasion of colorectal cells. Binding to IL-11 mRNA, LncRNA-ATB could upregulate IL-11 mRNA expression, enhance the mRNA stability of IL-11 and promote its secretion in colorectal cancer cells. Conclusions LncRNA-ATB may play an important role in the migration and invasion of colorectal cells by enhancing the secretion of IL-11.关键词
被转化生长因子β活化长链非编码RNA/白介素-11/侵袭/转移Key words
long non-coding RNA activated by TGF-β/interleukin-11/migration/invasion分类
医药卫生引用本文复制引用
李薇,孙锋,吴雪松,唐浩然,何水旺..lncRNA-ATB促进结肠癌细胞侵袭与转移的机制研究[J].中国现代医学杂志,2017,27(14):33-39,7.
