中国现代医学杂志2017，Vol.27Issue(14)：40-43,4.DOI:10.3969/j.issn.1005-8982.2017.14.008
脊髓CXCR2在CCI大鼠神经病理性疼痛中的作用研究
Role of spinal CXCR2 in neuropathic pain of rats suffered from chronic constriction injury
摘要
Abstract
Objective To explore the role of chemokine receptor 2 (CXCR2) of spinal cord in neuropathic pain of rats following sciatic nerve chronic constriction injury (CCI). Methods Eighteen adult male Sprague-Dawley rats were randomly divided into 3 groups (6 in each), i.e. N group (without any treatment), C group (CCI model) and T group (rats received intraperitoneal injection of 5 mg/kg Anacardic acid 3 d after CCI model establishment). Paw mechanical withdrawal threshold (PMWT) and paw thermal withdrawal latency (PTWL) were measured in the 3 groups 1 d before model establishment and on the 1st, 3rd, 5th and 7th d after CCI surgery. All lumbar spinal cords were dissected to examine the CXCR2 expression by Western blot. Results The PTWL of the CCI rats reduced from the 3rd d after surgery. Compared with the N group, the PTWL in the C and T groups were obviously reduced ( <0.05). Compared with the C group, the PTWL of the T group increased on the 5th and 7th d ( <0.05). The variation tendency of PMWT was similar to that of PTWL. Compared with the N group, the CXCR2 expression in the C and T groups increased ( <0.05). Compared with the C group, the CXCR2 expressions in the T group decreased ( <0.05). Conclusions Up-regulation of CXCR2 in the spinal cord may play an important role in the pathogenesis of neuropathic pain. Intraperitoneal injection of Anacardic acid could partly reduce the expression of CXCR2 in the lumbar spinal cord in CCI rats and relieve pain.关键词
神经病理性疼痛/趋化因子受体2/组蛋白乙酰化/表观遗传学Key words
neuropathic pain/chemokine receptor 2/histone acetylation/epigenetics分类
医药卫生引用本文复制引用
潘冰冰,肖丹,黄晓玲,魏来,唐轶珣,孔高茵..脊髓CXCR2在CCI大鼠神经病理性疼痛中的作用研究[J].中国现代医学杂志,2017,27(14):40-43,4.基金项目
湖南省自然科学基金青年基金(No:2017JJ3178) （No:2017JJ3178）
湖南省长沙市科技计划(No:K1401007-31) （No:K1401007-31）
湖南省人民医院2015年仁术基金 （）
