中国现代医学杂志2017，Vol.27Issue(14)：48-52,5.DOI:10.3969/j.issn.1005-8982.2017.14.010
MicroRNA-182在宫颈癌中的表达及对肿瘤细胞增殖、侵袭及迁移的影响
Expression of miR-182 in cervical cancer and its effect on proliferation, invasion and migration of cervical cancer cells
宋霞 1毛熙光1
作者信息
- 1. 西南医科大学附属医院 妇产科,四川 泸州 646000
- 折叠
摘要
Abstract
Objective To investigate the expression of miR-182 in cervical cancer, cervical intraepithelial neoplasia (CIN) and normal cervical tissue, and to discuss its effect on proliferation, invasion and migration of cervical cancer cells. Methods The expression level of miR-182 was detected by real-time PCR in 78 samples of cervical cancer tissues, 30 samples of CIN tissues and 20 samples of normal cervical tissues. The relationships between miR-182 expression and clinicopathological features of cervical cancer were analyzed. Cervical cancer CaSki cells were transfected with miR-182 mimics. CCK-8 assay and Transwell assay were used to analyze the effects of miR-182 on the proliferation, invasion and migration of cervical cancer cells. Results The expression level of miR-182 showed significant differences among the cervical cancer tissues, the CIN tissues and the normal cervical tissues ( <0.05). Pairwise comparisons showed the miR-182 expression in the cervical cancer tissues and the CIN tissues was significantly lower than that in the normal cervical tissues ( <0.05), but the difference between the cervical cancer tissues and the CIN tissues was not statistically significant ( >0.05). The expression level of miR-182 in the cervical cancer tissues was significantly correlated with degree of differentiation and lymphatic metastasis ( <0.05), but not in significant correlation with age, tumor diameter, depth of invasion or clinical stages ( >0.05). Transwell assay showed that up-regulated miR-182 expression would significantly reduce the migration and invasion of the cervical cancer CaSki cells ( <0.05). CCK-8 assay showed that up-regulated miR-182 expression had no effect on the proliferation of the cervical cancer cells ( >0.05). Conclusions The expression level of miR-182 is obviously declined in cervical cancer tissues and is related to degree of differentiation and lymphatic metastasis. The up-regulated miR-182 expression can inhibit the invasion and migration of cervical cancer CaSki cells.关键词
miR-182/宫颈癌/增殖/侵袭/迁移Key words
miR-182/cervical cancer/proliferation/invasion/migration分类
医药卫生引用本文复制引用
宋霞,毛熙光..MicroRNA-182在宫颈癌中的表达及对肿瘤细胞增殖、侵袭及迁移的影响[J].中国现代医学杂志,2017,27(14):48-52,5.
