中国现代医学杂志2017，Vol.27Issue(15)：1-4,4.DOI:10.3969/j.issn.1005-8982.2017.15.001
miR-30a-5p对肺癌细胞增殖与迁移的影响及其机制
miR-30a-5p inhibits proliferation and migration of non-small cell lung cancer cell and its mechanism*
摘要
Abstract
Objective To investigate the role of miR-30a-5p on the regulation of the proliferation and migration of NSCLC, and its regulatory mechanism. Methods A549 cells were divided into two groups, miR-30a-5p group (transfected with miR-30a-5p mimics) and control group (transfected with microRNA scrambles). The proliferation and migration ability was measured by MTT assay and wound healing experiment, respectively. The expression level of UBE3C mRNA and protein was measured by RT-PCR and Western blot. Results The relative percentage of living cells in miR-30a-5p group was significantly lower than control group after cultur-ing for 48 h, (203.7 ± 16.8)% vs (330.4 ± 20.7)%, =0.000. There was (258 ± 30.2)% of relative percentage of living cells in miR-30a-5p group, which was significantly lower than (403.4 ± 28.4)% in the control group ( =0.001). The wound healing rate was (23.2 ± 2.7%) in miR-30a-5p group, which was significantly lower than (89.2 ± 4.8)% in the control group ( =0.000). RT-PCR indicated that the expression level of UBE3C mRNA was (0.15 ± 0.02) in miR-30a-5p group, which was significantly lower than (1.03 ± 0.09) in the control group ( =0.000). The expression level of UBE3C protein was (1.57 ± 0.26) in miR-30a-5p group, which was signifi-cantly lower than (5.32 ± 0.42) in control group ( =0.000). Conclusions MiR-30a-5p inhibits cell prolifera-tion and migration of non-small cell lung cancer cell by suppressing UBE3C gene expression.关键词
miR-30a-5p/非小细胞肺癌/增殖/迁移Key words
miR-30a-5p/non-small cell lung cancer/proliferation/migration分类
医药卫生引用本文复制引用
邱会,胡敏,钟敏钰..miR-30a-5p对肺癌细胞增殖与迁移的影响及其机制[J].中国现代医学杂志,2017,27(15):1-4,4.基金项目
武汉市卫计委临床医学科研项目(No:WZ13Z03) （No:WZ13Z03）
