中国现代医学杂志2017，Vol.27Issue(15)：5-10,6.DOI:10.3969/j.issn.1005-8982.2017.15.002
整合素β1-黏附斑激酶在心肌纤维化中的作用及机制
Role and mechanism of integrin β1-FAK on myocardial fibrosis*
摘要
Abstract
Objective To study the effects and mechanisms of integrin β1-FAK on myocardial fibrosis. Methods Cells were randomly divided into five groups: the blank control group, the LPS-stimulated group, integrin β1 blocking group, FAK blocking group, blocking integrin β1 and FAK group. Double-immunostaining was used to test the purity of myocardial cells. RT-PCR and q-PCR were used to detect the gene expression of MMP-9, TGF-β, Col1A1, Col3A1 and integrin β1. Western blot method was used to detect the expression of FAK protein. Results Compared with those in the control group, the LPS-treated cells exhibited a significant increase of MMP-9, TGF-β, Col1A1, Col3A1 and integrin β1, while blocking the integrin β1 prevented the increase in LPS-induced up-regulation of MMP-9, TGF-β, Col1A1, Col3A1 and integrin β1 and inhibited the FAK phosphorylation. Furthermore, when the cells were treated with the FAK agonist, the expression of MMP-9 and TGF-β decreased, and it had similar effect with blocking integrin β1. Conclusions Our study suggests that inhibition of integrin β1 suppresses the FAK phosphorylation, and decreases expressons of MMP-9, TGF-β, Col1A1, Col3A1, which contributes to alleviate the development of myocardial fibrosis under the condition of continuous inflammation stimulation and provides new ideas for the future clinical treatment stimulated H9c2 cardiomyocytes.关键词
心肌纤维化/整合素/黏附斑激酶/血清脂肪酶Key words
myocardial fibrosis/integrin/focal adhesion kinase/lipase分类
医药卫生引用本文复制引用
林传钦,翟秀丽,邓托..整合素β1-黏附斑激酶在心肌纤维化中的作用及机制[J].中国现代医学杂志,2017,27(15):5-10,6.基金项目
三亚市医疗科技创新项目(No:YW1214) （No:YW1214）
