中国现代医学杂志2017，Vol.27Issue(15)：15-21,7.DOI:10.3969/j.issn.1005-8982.2017.15.004
肺癌细胞株中EPS8表达下降对顺铂化疗敏感性的影响
Effects of EPS8 expression decreased on cisplatin sensitivity in lung cancer cell
杜海坚 1李伟峰 1孙青峰 2王元亨 3黄文杰1
作者信息
- 1. 广州军区广州总医院 呼吸内科,广东 广州 510010
- 2. 广东药科大学,广东 广州 510006
- 3. 中国人民解放军第四军医大学,陕西 西安 710000
- 折叠
摘要
Abstract
Objective To evaluate the role of epidermal growth factor receptor pathway substrate 8 (EPS8) in cellular susceptibility to cisplatin in tumor cells and normal cells. Methods EPS8 RNA interference was used to determine EPS8 silencing efficiency in each cell line by qRT-PCR. Caspase-3/7 was used to detect the apoptosis of lymphoblastoid cell lines (LCLs) and A549 lung cancer cell when treated with 5 μmol/L cisplatin after EPS8 was silenced. In the Alamar Blue cell growth inhibition assay, 0.01 μmol/L mycophenolate as an EPS8 inhibitor was used alone or in combination with different concentrations of cisplatin to detected its effect on the growth inhibition of cancer and non-cancer cell lines. Results Compared with the control group, the survival rate of EPS8 in LCLs cell line was increased by 7.9% and the apoptosis rate decreased by 8.7%. In contrast, cisplatin intervention resulted in a 20.6% reduction in lung cancer cell survival. The expression of EPS8 was decreased in LCLs cell line, and the Caspase-3/7 activity was lower in the non-cancer cell line LCLs treated by cisplatin compared with the control group, and the apoptosis rate was decreased. In the five kinds of non-small cell lung cancer (NSCLC) cell lines, mithramycin also lead to decreased expression of EPS8. Compared with the LCLs cell line, the sensitivity to cisplatin increased significantly in the four NSCLC cells and the bladder cancer cell HTB9 ( <0.05), but it had no statistically significant in the NSCLC, the EGFR mutated H1975 cells ( >0.05). Conclusions There are significantly increased of sensitivity to cisplatin in cancer cells when EPS8 inhibition through the RNA interference or mithramycin treated,while normal cells decrease its sensitivity.关键词
顺铂/光神霉素A/敏感性/淋巴母细胞样细胞/非小细胞肺癌Key words
cisplatin/mithramycin/sensitivity/lymphoblastoid cells/non-small cell lung cancer分类
医药卫生引用本文复制引用
杜海坚,李伟峰,孙青峰,王元亨,黄文杰..肺癌细胞株中EPS8表达下降对顺铂化疗敏感性的影响[J].中国现代医学杂志,2017,27(15):15-21,7.
