中国现代医学杂志2017，Vol.27Issue(15)：22-28,7.DOI:10.3969/j.issn.1005-8982.2017.15.005
酪氨酸激酶3对赫赛汀耐药的卵巢癌细胞株SKOV3/H增殖和成瘤能力的影响
Effect of HER-3 on proliferation and tumorigenicity abilities of Herceptin-resistant ovarian cancer cell line SKOV3/H
耿介1
作者信息
- 1. 郑州大学第二附属医院 妇产科,河南 郑州 450014
- 折叠
摘要
Abstract
Objective To explore the role of HER-3 in ovarian cancer and Herceptin resistant ovarian cancer, and the effect of HER-3 on the proliferation abilities in vitro and tumorigenicity abilities in vivo of Herceptin-resistant ovarian cancer cell SKOV3/H. Methods Herceptin concentration was gradually increased to construct resistant cell lines (C0=5 mg/L, increased 5 to 8 times, each time increased by 50%). qRT-PCR was used to detect the expression of HER-3 in 15 pairs of ovarian cancer tissues which were non-resistant or resistant to Herceptin, and the expression of HER-3 in SKOV3 and SKOV3/H cells. MTT experiment was used to detect the proliferation of SKOV3/H after infected with sh-HER-3 or vector. KOV3/H cells infected with sh-HER-3 or vector were subcutaneously injected into nude mice to build the ovarian cancer xenografts. Tumor formation was observed in vivo situation. Results Successfully constructed the Herceptin-resistant ovarian cancer cell line SKOV3/H, the Td of SKOV3/H was 1.4 to 1.9 times of SKOV3. The expression levels of HER-3 was significantly higher in Herceptin resistant ovarian cancer tissues ( <0.05), and HER-3 expression levels were also significantly higher in SKOV3/H cells ( < 0.05). After transfected with sh-HER-3, the proliferation and tumorigenicity abilities of SKOV3/H were significantly lower than vector group ( <0.05). The tumorigenicity ability of SKOV3/H in mice was significantly reduced ( < 0.05). Conclusions HER- 3 is involved in the Herceptin resistant of ovarian cancer and affects the proliferation and in vivo tumorigenicity of SKOV3/H.关键词
卵巢癌/赫赛汀耐药/HER-3/增殖/体内成瘤Key words
ovarian cancer/herceptin resistant/HER-3/proliferation/tumorigenesis in vivo分类
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耿介..酪氨酸激酶3对赫赛汀耐药的卵巢癌细胞株SKOV3/H增殖和成瘤能力的影响[J].中国现代医学杂志,2017,27(15):22-28,7.
