中国现代医学杂志2017，Vol.27Issue(15)：29-33,5.DOI:10.3969/j.issn.1005-8982.2017.15.006
硝普钠与尼莫地平治疗大鼠脑出血损伤的疗效及与凝血酶受体-1表达的相关研究
Efficacy of nitroprusside and nimodipine in treatment of cerebral hemorrhage in rats and its correlation with thrombin receptor-1 expression
周燕利 1聂琼芳 1吴晓兰1
作者信息
- 1. 武汉理工大学医院 神经内科,湖北 武汉 430010
- 折叠
摘要
Abstract
Objective To investgate the effect of sodium nitroprusside and nimodipine on cerebral hemorrhage (ICH) in rats and its correlation with expression of thrombin receptor-1 (PAR1). Methods Thirty male Wistar rats (200-220 g) were randomly divided into 3 groups ( =10 each): sham operation group, cerebral hemorrhage group and combination therapy group. Rats were sacrificed 48 hours after successful ICH operation. The apoptosis of neurons was detected by TUNEL method. The water content of brain tissue was detected. The expression of thrombin receptor-1 (PAR1) protein was detected by Immunohistochemistry and Western blot. Results Compared with the sham group, the number of apoptotic cells and water content in cerebral hemor-rhage group and combination therapy group were significantly increased ( = 0.000), while the number of apoptotic cells and water content of brain tissue in combination group were significantly lower than the cerebral hemorrhage group ( =0.000). Compared with sham group, PAR1 protein expression in cerebral hemorrhage group (2.187 ± 0.233) and PAR1 protein expression in ICH group (1.434 ± 0.168) were significantly increased (0.673 ± 0.121) ( =0.000). Compared with ICH group, the PAR1 protein expression in the combination group significantly decreased ( =0.000), and the difference was statistically significant ( =0.000). The results of western blot were consistent with immunohistochemical results. Conclusions Sodium nitroprusside and nimodip-ine have neuroprotective effects on cerebral hemorrhage by regulating the expression of thrombin receptor-1 (PAR1) in rats.关键词
脑出血/硝普钠/尼莫地平/凝血酶受体-1Key words
cerebral hemorrhage/sodium nitroprusside/nimodipine/thrombin receptor-1分类
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周燕利,聂琼芳,吴晓兰..硝普钠与尼莫地平治疗大鼠脑出血损伤的疗效及与凝血酶受体-1表达的相关研究[J].中国现代医学杂志,2017,27(15):29-33,5.
