中国现代医学杂志2017，Vol.27Issue(15)：38-41,4.DOI:10.3969/j.issn.1005-8982.2017.15.008
自制爬片装置用于细胞爬片及细胞染色
New method of preparation cell-attached slides for staining with self-made device
苏获 1毛林锋 2Benson Kaluba 2段智曦 3刘擎 2黄鹏2
作者信息
- 1. 中南大学护理学院,湖南 长沙 410013
- 2. 中南大学湘雅医院,湖南 长沙 410008
- 3. 中南大学湘雅二医院,湖南 长沙 410011
- 折叠
摘要
Abstract
Objective To provide an improved method to prepare cell-attached slides. Methods Cell-attached slides were prepared by routine method and self-made device, and the difference between the two methods were compared by observing the cell growth after HE staining. Three kinds of interventions were given to the same cell-attached slide to test the application in making different interventions cell-attached slide of the device. Three kinds of cells were planted in the same slide to test the application in making integrated cell-attached slides of the device. Results Our device simplified the preparation of cell-attached slides. Cells grown on the glass and never showed pollution or growth restriction, given different interventions or planted different cells at the same time without interaction. Conclusions The new method simplifies the processes of preparation cell-attached slides, and the slides made by our device satisfies the experiment demands of all kinds of cells staining, makes it as a more effective method than before.关键词
细胞爬片/细胞培养/染色Key words
cell-attached slide/cells culture/cells staining分类
生物科学引用本文复制引用
苏获,毛林锋,Benson Kaluba,段智曦,刘擎,黄鹏..自制爬片装置用于细胞爬片及细胞染色[J].中国现代医学杂志,2017,27(15):38-41,4.
