摘要
Abstract
Objective To investigate the endothelin receptor A, B (ETAR, ETBR) expression in renal tis-sue of the development in elder diabetic nephropathy. Methods From January 2015 to June 2016, 34 elderly patients with diabetic nephropathy in our hospital were divided into 3 groups according to pathological type:10 cases of group D1 (12 cases), grade D2 group (12 cases), grade D3 group. Patients underwent renal tumor resection from renal tumors in our hospital as the control group (10 cases). The expressions of ETAR and ETBR were detected by immunohistochemistry. The location of ETAR and ETBR in renal tissue was detected by immunofluorescence double staining method. Biochemical detection indexes were collected to calculate the rate of glomerular sclerosis, and the correlation was analyzed. Results The urine protein level of D3 group was significantly higher than that of D2 and D1 group, the difference was statistically significant ( <0.05). Glomerular filtration rate (GFR) and albumin (Alb) had significant difference between group D3 and D1 ( <0.05). D3 group and D2 group glomerular sclerosis rate were significantly higher than that in D1 group, the difference had statistical difference ( < 0.05). The expression of ETAR and ETBR in diabetic nephropathy group were significantly higher than the control group, the difference was statistically significant ( <0.05). The expression of ETAR was less than ETBR in the control group, with the aggravation of increased expression of ETAR in renal tissue of diabetic nephropathy patients, the differences between groups were statistically significant ( <0.05). The expression of ETBR in D2 group was the highest, and no statistically significant differences between D1 group and D3 group ( >0.05). ETAR/ETBR in each group had statistically significant difference ( <0.05). ETAR was expressed in proximal and distal renal proximal tubule. ETBR was mainly expressed in proximal tubule. The expression of ETAR in renal tissue of patients with diabetic nephropathy and duration, urinary protein level and ROG were positively correlated. The expression of ETBR was negatively related to the amount and duration. ETAR/ETBR ratio and the course of disease, urinary protein level, ROG were positively correlated, negatively correlated with Alb, GFR. Conclusions The expression of ETAR and ETBR in the renal tissues of the elderly patients with diabetes mellitus are significantly increased, and the inversion of ETAR/ETBR value is positively correlated with the development of the disease.关键词
糖尿病肾病/肾组织/内皮素受体/老年人Key words
diabetic nephropathy/renal tissue/endothelin receptor/elder分类
医药卫生
