中国现代医学杂志2017，Vol.27Issue(16)：1-5,5.DOI:10.3969/j.issn.1005-8982.2017.16.001
姜黄素通过上皮-间质转化过程对口腔鳞癌细胞株 HN4侵袭转移的影响
Effect of curcumin on invasion and metastasis of oral squamous cell carcinoma HN4 cell line by EMT
摘要
Abstract
Objective To investigate the effect of curcumin on proliferation and metastasis of oral squamous cell carcinoma HN4 cell line and the molecular mechanism. Methods Oral squamous cell carcinoma HN4 cells were cultured and treated with 0-60 μmol/L curcumin, and MTT was used to assess the cell viability after 24 hours. Wound scratch assay was performed to detect the invasion of the HN4 cells. Cell cycle and apoptosis were detected by flow cytometry. The protein expression levels of p53, E-cadherin and Snail were detected by Western blot. Results MTT assay revealed that curcumin significantly inhibited the growth of the cancer cells at the experimental concentrations, the effect was more obvious at the concentration of 20 μmol/L. With the increase of curcumin concentration, the effect on cell apoptosis was getting more significant. The HN4 cells were arrested at G0/G1 phase after treatment. With the increase of curcumin concentration, the expression of Snail protein decreased, and the expressions of p53 and E-cadherin increased. Conclusions Curcumin can significantly inhibit the growth of oral squamous cell carcinoma HN4 cell line, and the mechanism may be up-regulation of p53 expression.关键词
姜黄素/口腔鳞癌/凋亡Key words
curcumin/oral squamous cell carcinoma/apoptosis分类
医药卫生引用本文复制引用
张佳,黄汉,张斌,王志红..姜黄素通过上皮-间质转化过程对口腔鳞癌细胞株 HN4侵袭转移的影响[J].中国现代医学杂志,2017,27(16):1-5,5.基金项目
辽宁省科技厅自然科学基金资助项目(No:2015020333) （No:2015020333）
