中国现代医学杂志2017，Vol.27Issue(16)：6-11,6.DOI:10.3969/j.issn.1005-8982.2017.16.002
氧化芍药苷对泡沫细胞胆固醇流出的影响
Effect of Oxypaeoniflora on cholesterol efflux from foam cells
唐菀泽 1马卫列 1丁航 1向乐 1张志珍1
作者信息
- 1. 广东医科大学 生物化学与分子生物学教研室,广东 东莞 523808
- 折叠
摘要
Abstract
Objective To analyze the effect of Oxypaeoniflora on cholesterol efflux from THP-1 macrophage-derived foam cells. Methods THP-1 cells were treated with 160 nmol/L phorbol-1-myristate-13-acetate (PMA) for 24 h, then 50 μg/ml acetylated low-density lipoprotein (ac-LDL) to establish foam cell model. Cytotoxicity of Oxypaeoniflora was assessed by CCK-8. Cholesterol efflux rate of the foam cells in each treatment group was evaluated by isotope labeling method. The expression of adipose differentiation related protein (ADFP) in the foam cells was observed by confocal laser scanning microscopy. The expression changes of ADFP, ATP binding cassette transporter A1 (ABCA1) and peroxisome proliferator-activated receptor alpha (PPARα) in the foam cells were detected using Western blot. Results The THP-1 macrophage-derived foam cell model was successfully established. At the concentration of lower than 200.0 μg/ml, Oxypaeoniflora was not cytotoxic. When the Oxypaeoniflora concentrations were 150.0 μg/ml and 200.0 μg/ml, the cholesterol efflux rates were about (10.317 ± 0.508)% and (11.265 ± 0.713)% respectively, which were increased compared with the control group ( < 0.05). Under microscope, the fluorescence of ADFP in the Oxypaeoniflora groups was obviously decreased. Western blot indicated that the expression of ADFP was decreased by 28%, while the expressions of PPARαand ABCA1 were increased by 51% and 45% respectively. Conclusions The results state clearly Oxypaeoniflora can increase the expression of ABCA1 by up-regulation of PPARαexpression and then promote the cholesterol efflux from foam cells.关键词
氧化芍药苷/THP-1巨噬细胞源性泡沫细胞/胆固醇流出Key words
Oxypaeoniflora/THP-1 macrophage-derived foam cell/cholesterol efflux分类
医药卫生引用本文复制引用
唐菀泽,马卫列,丁航,向乐,张志珍..氧化芍药苷对泡沫细胞胆固醇流出的影响[J].中国现代医学杂志,2017,27(16):6-11,6.
