中国现代医学杂志2017，Vol.27Issue(16)：18-23,6.DOI:10.3969/j.issn.1005-8982.2017.16.004
供体来源半成熟树突状细胞诱导大鼠肝移植 术后免疫低反应的实验研究
Induction of low immune response by donor-derived semi-mature dendritic cells in rats after liver transplantation
摘要
Abstract
Objective To study the effect of donor-derived semi-mature dendritic cells (smDC) in inducing low immune response of rats after orthotopic liver transplantation and analyze its mechanism. Methods In this experiment, based on culture and identification of rat dendritic cells, donor-derived dendritic cells were infused before orthotopic liver transplantation, and Rapamycin was used for gastric lavage after surgery. Then the expression levels of cytokines (IL-10 and IFN-γ) and CD4+CD25+Treg changes were observed, and the pathological changes of acute immunological rejection were evaluated. Results Administration of Rapamycin changed serum cytokine secretion, promoted CD4 +CD25 +Treg proliferation after liver transplantation in the Lewis→BN rats, alleviated postoperative immune rejection, thus prolonged the survival time after liver transplantation. Preoperative infusion of immature dendritic cells (imDC) or smDC could enhance the effect of Rapamycin and further induce the low immune response after liver transplantation in rats, and the effect of smDC was stronger than that of imDC. Conclusions smDC can induce low immune response after liver transplantation of Lewis→BN rats.关键词
肝移植/半成熟树突状细胞/免疫排斥/调节性T细胞Key words
liver transplantation/semi-mature dendritic cell/immune rejection/regulatory T cell分类
医药卫生引用本文复制引用
李春满,王伟,张捷,唐波,李刚,董丽英,朱磊,付必莽..供体来源半成熟树突状细胞诱导大鼠肝移植 术后免疫低反应的实验研究[J].中国现代医学杂志,2017,27(16):18-23,6.基金项目
云南省卫生科技计划项目(No:2014NS063) （No:2014NS063）
云南省科技计划项目(No:2014FB053) （No:2014FB053）
