中国现代医学杂志2017，Vol.27Issue(16)：30-34,5.DOI:10.3969/j.issn.1005-8982.2017.16.006
雌激素对兔脊髓缺血再灌注损伤保护作用的机制研究
Protective effect of estrogen on spinal cord ischemia-reperfusion injury in rabbits
刘红光 1张超 1曾愈程 1刘芳 1康颖2
作者信息
- 1. 南华大学附属第一医院 乳腺甲状腺外科,湖南 衡阳 421001
- 2. 南华大学医学院 外科学教研室,湖南 衡阳 421001
- 折叠
摘要
Abstract
Objective To evaluate the protective effect of estrogen on spinal cord ischemia-reperfusion injury in rabbits and its mechanism. Methods According to a random number table method, adult New Zealand rabbits were divided into A-E five groups with 9 in each group. In the rabbits of group A, infrarenal aorta was clamped for 20 min and then blood circulation was restored (reperfusion). The rabbits of group B received the same anesthesia and surgery as group A, but no ischemia-reperfusion injury. The rabbits of groups C, D and E had injection of estrogen 200, 400 and 800 μg/kg respectively through ear veins when reperfusion started. The neurolo gical function of the lower extremities was scored according to Tarlov law 48 h after reperfusion. Then hematoxylin-eosin staining was used for pathological analysis of spinal cord. Results The neurological function scores of the groups C, D and E were significantly higher than those of the group A ( < 0.05). Histopathological study showed apoptosis and marked necrosis of the motor neurons in the anterior horn of the spinal cord. The number of anterior horn motor neurons in the group A was significantly smaller than that of the estrogen groups ( <0.05). Conclusions Estrogen can significantly improve the neurological function of the lower limbs of rabbits with spinal cord ischemia and reperfusion, increase the number of normal motor neurons in the anterior horn of the spinal cord, and reduce ischemia-reperfusion injury.关键词
雌激素/脊髓缺血/再灌注损伤Key words
estrogen/spinal cord ischemia/reperfusion injury分类
医药卫生引用本文复制引用
刘红光,张超,曾愈程,刘芳,康颖..雌激素对兔脊髓缺血再灌注损伤保护作用的机制研究[J].中国现代医学杂志,2017,27(16):30-34,5.
