中国现代医学杂志2017，Vol.27Issue(16)：35-39,5.DOI:10.3969/j.issn.1005-8982.2017.16.007
热性惊厥患儿血清中表观遗传标志物的 表达及临床意义
Expression and significance of epigenetic markers in serum of children with febrile seizures
摘要
Abstract
Objective To explore the expression and clinical significance of epigenetic markers in the serum of the children with febrile seizures (FS). Methods RT-PCR and ELISA were used to measure the gene and protein ex-pressions of DNA methyltransferase 1 (DNMT1), DNMT3A, DNMT3B, histone methyltransferase EZH2 (EZH2), and histone deacetylase 4 (HDAC4) in 60 children with febrile seizures [30 with simple FS (SFS) and 30 with complex FS (CSF)] and 30 healthy controls. Results The gene expression levels of DNMT1, DNMT3A, DNMT3B, EZH2 and HDAC4 in the SFS and CFS groups were significantly higher than those in the control group ( < 0.05), while there were no significant differences between the SFS group and the CFS group ( > 0.05). The protein expressions of DNMT1, DNMT3A, DNMT3B, EZH2 and HDAC4 in the SFS and CFS groups were significantly higher than those in the control group ( < 0.05); there were no significant differences between the SFS group and the CFS group ( >0.05). Conclusions DNMT1, DNMT3A, DNMT3B, EZH2 and HDAC4 are highly expressed at the gene and protein levels in the serum of the children with SFS and CFS, which suggests that these epigenetic modifications may play important roles in the occurrence and development of FS and provides a reliable theoretical basis for further research of pathogenesis and prevention of FS.关键词
热性惊厥/表观遗传/DNA甲基转移酶/组蛋白甲基化酶/组蛋白去乙酰化酶Key words
febrile seizure/epigenetic/DNA methyltransferase/histone methyltransferase/histone deacetylase分类
医药卫生引用本文复制引用
徐健,孙明强,王媛媛,张成元..热性惊厥患儿血清中表观遗传标志物的 表达及临床意义[J].中国现代医学杂志,2017,27(16):35-39,5.基金项目
国家自然科学基金(No:81401230) （No:81401230）
潍坊市科学技术发展计划项目(No:2014001) （No:2014001）
