中国畜牧兽医2017，Vol.44Issue(6)：1580-1587,8.DOI:10.16431/j.cnki.1671-7236.2017.06.002
山羊CMKLR1基因的克隆、表达及其与肌内脂肪的相关性研究
Cloning and Expression of CMKLR1 Gene and Its Correlation with Intramuscular Fat in Goat (Capra hirus)
摘要
Abstract
In order to enrich basic date in goat (Capra hirus) CMKLR1 gene and investigate the correlation between CMKLR1 gene expression and intramuscular fat (IMF) content in muscles, the CMKLR1 gene was cloned from the goat of subcutaneous adipose tissue by RT-PCR, characterized by bioinformatics methods.The expression profiles of CMKLR1 gene of goat in various tissues were constructed liver was benchmark, the GAPDH (GenBank accession No.: AJ431207.1) as a reference gene.Then, the correlation between CMKLR1 mRNA expression and IMF content in muscles was analyzed.The results showed that the CMKLR1 gene CDS of goat (GenBank accession No.: KT165374) was 1 089 bp.Real-time PCR indicated that CMKLR1 was widely expressed in various tissues, including heart, liver, spleen, lung, kidney, adipose, longissimus dorsi, biceps femoris and triceps brachii, and was the highest in lung (P<0.05).Similar expression variation trend of CMKLR1 was observed between the muscles from 1 to 3 and 24 months old goat, and was the highest in biceps femoris.Reversely, in 8 to 10 months old CMKLR1 was the highest in triceps brachii.The IMF of longissimus dorsi from 24 months old was the highest.Correlation analysis demonstrated that different correlations were observed between expression of CMKLR1 mRNA and IMF content in longissimus dorsi, biceps femoris, and triceps brachii in goat.The CMKLR1 gene did not participate in the deposition of IMF in goats.The research built the theoretical basis for further studies about the CMKLR1 gene.关键词
山羊/CMKLR1基因/克隆/表达谱/肌内脂肪(IMF)Key words
goat/CMKLR1 gene/clone/expression profile/intramuscular fat (IMF)分类
农业科技引用本文复制引用
廖红海,林亚秋,朱江江,王永..山羊CMKLR1基因的克隆、表达及其与肌内脂肪的相关性研究[J].中国畜牧兽医,2017,44(6):1580-1587,8.基金项目
国家自然科学基金(31672395、31601921) （31672395、31601921）
四川省"十三五"畜禽育种攻关项目(2016NZ0099-36) （2016NZ0099-36）
四川省科技创新产业链示范工程重大项目(2014NZ0003) （2014NZ0003）
