中国畜牧兽医2017，Vol.44Issue(6)：1596-1603,8.DOI:10.16431/j.cnki.1671-7236.2017.06.004
猪JHDM2A基因的克隆及表达分析
Cloning and Expression Analysis of Porcine JHDM2A Gene
摘要
Abstract
The aim of this study was to clone the JHDM2A gene of porcine and study the expression of JHDM2A gene in porcine ovary.In this study, we cloned the porcine JHDM2A gene and constructed its eukaryotic expression vector, the expression of JHDM2A gene in porcine ovarian tissue was also analyzed.The results showed that the cloned CDS length of porcine JHDM2A gene was 3 945 bp, which encoded 1 315 amino acids.The results of multiple amino acid sequence comparison showed that the porcine JHDM2A shared 93.5%, 94.7%, 94.7% and 93.8% homologous compared with those of Bubalus bubalis, Bos taurus, Ovis aries and Homo sapiens, respectively.Phylogenetic tree analysis indicated that the JHDM2A gene was highly conservative in the evolutionary process.The pLVX-IRES-ZsGreen1-JHDM2A eukaryotic expression vector was constructed, and clear green fluorescent signal was observed when the plasmid was transfected into HEK293T cells by liposome method.The immunohistochemical results showed that the JHDM2A protein was expressed in porcine follicle of different development stages.The results showed that the porcine JHDM2A gene sequences was cloned, and the JHDM2A protein was highly expressed in porcine ovary, indicating that its function might be closely related to the development of porcine follicular.关键词
猪/JHDM2A基因/克隆/卵泡/表达分析Key words
porcine/JHDM2A gene/cloning/follicle/expression analysis分类
生物科学引用本文复制引用
宋延霞,吴大平,李海艳,李胜,粟小平,李湘萍..猪JHDM2A基因的克隆及表达分析[J].中国畜牧兽医,2017,44(6):1596-1603,8.基金项目
国家自然科学基金(31560632) （31560632）
广西自然科学基金项目(2014GXNSFAA118099、2016GXNSFDA380030) （2014GXNSFAA118099、2016GXNSFDA380030）
