中国畜牧兽医2017，Vol.44Issue(6)：1621-1629,9.DOI:10.16431/j.cnki.1671-7236.2017.06.007
6株猪流行性腹泻病毒贵州株S基因序列分析
Sequence Analysis of S Gene of 6 Strains of Porcine Epidemic Diarrhea Virus from Guizhou
摘要
Abstract
In order to understand the characteristics of S gene of porcine epidemic diarrhea virus (PEDV) in Guizhou province, and grasp the genetic variation of PEDV, according to 3 pairs of specific primers designed in the test, S gene of 6 strains of PEDV from Guizhou were amplified by RT-PCR, cloned and sequenced.Nucleotide sequence and phylogenetic tree of 6 strains of PEDV from Guizhou and reference strains were analyzed by biological information software.The result showed that the length of S gene genome of 6 strains of PEDV from Guizhou was 4 161 bp, encoding 1 387 amino acids, and the nucleotide homologies of domestic and foreign PEDV reference were from 93.3% to 98.8%, the amino acid identity were from 91.6% to 98.9%.Phylogenetic tree analysis results showed that the 6 strains were close to the American strain, Vietnam strain and the Shandong strain.They were far from the CV777, LZC, Brl and 83P-5.The experiment showed that the S gene of PEDV in Guizhou had a certain degree of amino acid change in recent year with a trend of variation.The study provided a theoretical basis for the prevention and control of PEDV in Guizhou province.关键词
猪流行性腹泻/S基因/生物信息学分析Key words
porcine epidemic diarrhea/S gene/bioinformatics analysis分类
农业科技引用本文复制引用
毛黎红,张双翔,周碧君,王伟,胡兴义,程振涛,文明,王开功..6株猪流行性腹泻病毒贵州株S基因序列分析[J].中国畜牧兽医,2017,44(6):1621-1629,9.基金项目
贵州省科学技术厅农业攻关项目"贵州省仔猪腹泻性疫病风险分析及综合防控技术研究"(黔科合NY[2015]3009-1号) （黔科合NY[2015]3009-1号）
贵州省生猪质量安全工程技术研究中心建设项目"生猪健康养殖实验室建设与技术研发"黔科合农C字[2011]4022号 （）
贵州省农业委员会科技计划项"2015年贵州省重大动物疫病省级定点监测"[2015-11号] （）
