中国畜牧兽医2017，Vol.44Issue(6)：1657-1665,9.DOI:10.16431/j.cnki.1671-7236.2017.06.012
日粮能量及蛋白质水平对滩羊糖类转运载体mRNA表达的影响
Effects of Diets with Different Energy and Protein Levels on mRNA Expression of Glucose Transporters in Tan Sheep
摘要
Abstract
This experiment was conducted to study the effects of the diets with different energy and protein levels on mRNA expression of glucose transporters in the small intestine and muscle of Tan sheep.A total of 112 healthy Tan sheep (half male and half female) with similar initial live weight were randomly divided into four groups with four replicates per group and seven sheep per replicate.According to the Feeding Standard of Meat-producing Sheep and Goats (NY/T 816-2004),each group was fed diet with different levels of energy and protein respectively:0.84×standard level (group Ⅰ),0.96×standard level (group Ⅱ),1.08×standard level (group Ⅲ) and 1.20×standard level (group Ⅳ).The test period were divided into two stages by body weight of sheep (29-35 and 36-40 kg).At the end of each stage,one sheep was slaughtered at each replicate,and small intestine and muscle samples were collected to study mRNA relative expression of SGLT1,GLUT4 and GLUT5 genes by Real-time PCR.The results indicated that:SGLT1 gene mRNA expression levels of group Ⅲ was significantly higher than other groups in small intestine at the end of 29-35 kg stage (P<0.05);At the end of 36-40 kg stage,the SGLT1 gene mRNA expression levels had no significant difference among the four groups (P>0.05).In muscle,the SGLT1 gene mRNA expression level increased with the rise of energy and protein levels at both stages ,and that of group Ⅳ were the highest.In small intestine,at the end of 29-35 kg stage,the GLUT4 gene mRNA expression levels had no significant difference among the four groups (P>0.05);At the end of 36-40 kg stage,the GLUT4 gene mRNA expression level increased with the rise of energy and protein levels,and that of group Ⅳ were significantly higher than the other groups (P<0.05).In muscle,the GLUT4 gene mRNA expression level were the highest in group Ⅳ at both stages,and significantly higher than group Ⅰ (P<0.05).The GLUT5 gene mRNA expression did not show any regularity at both stages.In conclusion,diets with different levels of energy and protein could significantly affect the mRNA expression of SGLT1 and GLUT4 genes,and affect absorption of glucose in sheep.关键词
滩羊/能量/蛋白质/葡萄糖转运载体Key words
Tan sheep/energy/protein/glucose transporters分类
农业科技引用本文复制引用
卢艳娟,王小芳,曾洁,陈玉林,张恩平..日粮能量及蛋白质水平对滩羊糖类转运载体mRNA表达的影响[J].中国畜牧兽医,2017,44(6):1657-1665,9.基金项目
国家绒毛用羊产业技术体系(CARS-40-13) （CARS-40-13）
公益性行业(农业)科研专项(201303059) （农业）
陕西省科学技术研究发展计划项目(2014K01-17-03) （2014K01-17-03）
陕西省农业科技攻关项目(2014K01-17-04) （2014K01-17-04）
