中国畜牧兽医2017，Vol.44Issue(6)：1762-1768,7.DOI:10.16431/j.cnki.1671-7236.2017.06.026
70周龄长顺绿壳蛋鸡HO-1基因在不同组织中表达差异研究
Study on Different Expression of HO-1 Gene of 70-weeks-old Changshun Blue-shelled Chicken in Different Tissues
摘要
Abstract
In order to study the different expression of HO-1 gene in different tissues of 70-weeks-old Changshun Blue-shelled chickens.We collected nine (3 of each genotype) Changshun Blue-shelled chickens of 70-weeks-old as samples.The brain, liver, kidney, ovary, fallopian tube and uterus were collected, and total RNA of every tissue was extracted and reversed transcription to cDNA.The expression of HO-1 mRNA in different samples were detected by quantitative Real-time PCR.We compared the expression in the same tissues of different genotypes and in the different tissues of the same genotype.The results suggested that there were differences among different tissues of the same genotypes and different genotypes of the same tissues.The expression of HO-1 gene was highest in the liver, and there were significant difference in different genotype (P<0.01).The expression level in the uterus of White-shelled chicken was extremely significantly higher than other two genotypes (P<0.01),there was no significant difference between homozygous and heterozygous Blue-shelled chickens (P>0.05).关键词
长顺绿壳蛋鸡/HO-1基因/表达/实时荧光定量PCRKey words
Changshun Blue-shelled chicken/HO-1 gene/expression/quantitative Real-time PCR分类
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卜小雁,李辉,施晓丽,师新彩,赵忠海,陈林..70周龄长顺绿壳蛋鸡HO-1基因在不同组织中表达差异研究[J].中国畜牧兽医,2017,44(6):1762-1768,7.基金项目
长顺县厅地县合作项目"长顺绿壳蛋鸡绿壳基因的分子辅助选育"[CKH(2015)12] （2015）
