中国畜牧兽医2017，Vol.44Issue(6)：1769-1777,9.DOI:10.16431/j.cnki.1671-7236.2017.06.027
粤北地区新生仔猪腹泻病原学调查及PEDV ORF3基因分子特征和遗传变异分析
Etiological Investigation of Diarrhea in Newborn Piglets and Molecular Characterization and Genetic Variation Analysis of ORF3 Gene of PEDV in North Guangdong
摘要
Abstract
In order to understand the main pathogen of newborn piglets diarrhea in North Guangdong region, 31 diarrhea samples were collected from six pig farms in North Guangdong, the pathogen of porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PoRV) were detected by Real-time RT-PCR, meanwhile, ORF3 gene of PEDV amplified from positive samples were sequenced and analyzed.Pathogen detection results showed that 83.87%(26/31) samples were positive for PEDV, all herds and samples were negative for TGEV and PoRV.The sequence analysis revealed that the ORF3 gene of 5 epidemic strains of PEDV were all 675 bp, homologies of nucleotides were 98.7% to 100.0%, and homologies with reference sequences of nucleotides were 94.5% to 100.0%, and some gene mutation in common nucleotides site.The results of gene phylogenetic trees showed that PEDV could be divided into two groups, PEDV genetic relationship between field strains in North Guangdong and some regions in China, Southeast Asia, North America, Europe from 2013 to 2015 was closer, classed as a gene subgroup, from 2011 to 2012 main epidemic strains in our country and vaccine strains classed as other two gene subgroups.These results indicated that PEDV infection was the main pathogen of newborn piglets diarrhea in North Guangdong region, as time passed, the PEDV epidemic strains gene presented a tendency of evolution and variation.关键词
新生仔猪腹泻/病原学/猪流行性腹泻病毒/ORF3基因/遗传变异Key words
diarrhea in newborn piglets/etiology/porcine epidemic diarrhea virus/ORF3 gene/genetic variation分类
农业科技引用本文复制引用
南文金,胡鸿惠,吴静波,黄健强,彭国良,肖正中..粤北地区新生仔猪腹泻病原学调查及PEDV ORF3基因分子特征和遗传变异分析[J].中国畜牧兽医,2017,44(6):1769-1777,9.基金项目
广东省科技计划项目(2016A040402038、2013B020306008) （2016A040402038、2013B020306008）
广东省现代农业科技创新联盟建设项目(2016LM1141) （2016LM1141）
韶关市科技计划项目(2013CX/N09) （2013CX/N09）
韶关学院校级科研项目(SY2016KJ24) （SY2016KJ24）
