中国畜牧兽医2017，Vol.44Issue(6)：1811-1817,7.DOI:10.16431/j.cnki.1671-7236.2017.06.033
奶牛乳房炎病原菌诊断技术研究进展
Research Progress on Diagnosis Technology of Dairy Cow Mastitis Pathogens
摘要
Abstract
Dairy cow mastitis is one of the most costly diseases affecting the development of the world dairy industry.It causes a serious economic loss to dairy farming, and affects the yield and quality of milk, endangers human health.The cause of dairy cow mastitis is complex, but the main cause is pathogen infection, particularly Staphylococcus, Streptococcus and intestinal bacterial infection.The rapid diagnosis of pathogenic bacteria of mastitis is crucial for the effectively treatment of the dairy cow mastitis.In order to provide theoretical basis for developing rapid, specific and sensitive of dairy cow mastitis pathogens diagnosis, the author summarized the microbiological and biochemical assay method in routine used of dairy cow mastitis pathogen diagnosis, automatic microbial identification system, resazurin trace plate method, immune diagnosis method, the nucleic acid probe hybridization, 16S rRNA gene sequences of sequencing, PCR, gene chip and LAMP etc, and analyzed the advantages and disadvantages of these methods.关键词
奶牛/乳房炎/病原菌/诊断技术Key words
dairy cow/mastitis/pathogenic bacteria/diagnosis technology分类
农业科技引用本文复制引用
王丹,杨峰,李新圃,罗金印,刘龙海,张哲,张亚茹,张莉莉,李宏胜..奶牛乳房炎病原菌诊断技术研究进展[J].中国畜牧兽医,2017,44(6):1811-1817,7.基金项目
"十二五"国家科技支撑计划(2012BAD12B03) （2012BAD12B03）
甘肃省科技支撑计划(144NKCA240) （144NKCA240）
甘肃省农业科技创新项目(GNCX-2013-59) （GNCX-2013-59）
中国农业科学院奶牛疾病研究创新工程项目(CAAS-ASTIP-2014-LIHPS-03) （CAAS-ASTIP-2014-LIHPS-03）
