中国畜牧兽医2017，Vol.44Issue(6)：1832-1839,8.DOI:10.16431/j.cnki.1671-7236.2017.06.036
辽宁地区奶牛乳房炎源大肠杆菌毒力基因及耐药基因检测分析
Detection and Analysis of Virulence Genes and Drug Resistance Genes in E.coli Strains Isolated from Dairy Cows with Clinical Mastitis in Liaoning Region
摘要
Abstract
In order to understand the virulence genes and drug resistance genes carried by E.coli strains from dairy cows with mastitis in large-scale dairy farms in Liaoning region, and also provide improved programs for control and treatment of mastitis to dairy farmers, 66 strains of E.coli isolated from milk of dairy cows with clinical mastitis in several large-scale farms in Liaoning region were examined to detect 4 virulence genes and 4 drug resistance genes using PCR methods.The results showed that none of the target genes was detected in only one strain, while at least 2 and up to 7 target genes were detected in the rest of 65 strains.The detection rates of the virulence genes stx2e, eaeA, K99 and astA were 56.1%, 47.0%, 34.8% and 31.8%, respectively.In addition, the detection rate of dual virulence gene reached 43.9%, in which the genotype with the highest detection rate was eaeA/stx2e.The detection rates of the drug resistance genes sul3, sul1, cmlA and aacA4 were 87.9%, 83.3%, 40.9% and 28.8%, respectively.And the dual resistance gene detection rate was 36.4%, in which the highest detection rate was sul1/sul3 genotype;Triple drug resistance gene detection rate was 37.9%, and cmlA/sul1/sul3 presented the highest detection rate.These results confirmed that the E.coli isolated from dairy cow mastitis in large-scale dairy farms in Liaoning region had high detecting rates of sulfonamide resistance genes and chloramphenicol resistance genes, which was directly related to the drug resistance of the E.coli.These results provided important guiding significance for the prevention and control of dairy cow mastitis in Liaoning region as well as the safety of public health.关键词
奶牛乳房炎/大肠杆菌/毒力基因/耐药基因Key words
dairy cow mastitis/E.coli/virulence genes/drug resistance genes分类
农业科技引用本文复制引用
姚伟,高锋,曹明慧,顾贵波,赵晓彤,于学武,陈瑶,申贯男,魏园园..辽宁地区奶牛乳房炎源大肠杆菌毒力基因及耐药基因检测分析[J].中国畜牧兽医,2017,44(6):1832-1839,8.基金项目
辽宁省博士启动基金(201501119) （201501119）
