中国畜牧兽医2017，Vol.44Issue(6)：1847-1853,7.DOI:10.16431/j.cnki.1671-7236.2017.06.038
数字RT-PCR技术检测H3N2亚型猪流感病毒方法的建立
Establishment of Digital RT-PCR Assay for Detection of Swine Influenza Virus H3N2 Subtype
摘要
Abstract
A new assay for the detection of swine influenza virus (SIV) was developed with a novel nucleic acid probe——Molecular beacon in this study.The specific primers and molecular beacon probes were designed according to the conserved region of H3 and N2 genes of SIV H3N2 subtype.A digital RT-PCR assay was developed for detection of SIV H3N2 subtype.The results showed that SIV H3N2 subtype could be identified simultaneously on this microarry with high sensitivity and reproducibility,which could reach to 106 dilute viruse.The conclusion was that the digital RT-PCR method could analyze quantitatively the RNA templates.On the identification of H3N2 SIV,the digital RT-PCR method was much more scientific than Real-time quantitative PCR method.关键词
猪流感病毒/H3N2亚型/分子信标探针/数字RT-PCR/实时荧光定量PCR/灵敏性/特异性/重复性Key words
swine influenza virus/H3N2 subtype/molecular beacon probe/digital RT-PCR/Real-time quantitative PCR/sensitivity/specificity/repeatability分类
农业科技引用本文复制引用
唐连飞,禹思宇,周宇..数字RT-PCR技术检测H3N2亚型猪流感病毒方法的建立[J].中国畜牧兽医,2017,44(6):1847-1853,7.基金项目
国家质检总局科技资助项目(2014IK243) （2014IK243）
