中国畜牧兽医2017，Vol.44Issue(6)：1861-1868,8.DOI:10.16431/j.cnki.1671-7236.2017.06.040
伊维菌素微乳中伊维菌素的含量测定方法研究
Content Determination of Ivermectin in the Ivermectin Microemulsion Injection
摘要
Abstract
In order to establish the determination method of ivermectin (IVM) in the ivermectin microemulsion injection by high performance liquid chromatography (HPLC), Hypersil ODS2 colunm (5 μm,4.6 mm×250 mm) was used in this study.The mobile phase composed of methanol, acetonitrile and water (35∶60∶5,V/V/V) at a flow rate of 1 mL/min.The detection wave length was set at 244 nm and the column temperature was 30 ℃.The results showed that the HPLC system suitability of IVM was good.A good linear correlation of IVM was observed within the concentration range 80 to 320 μg/mL, and the average recovery rate was 101.90%±2.94% with RSD was 2.88%, the regression equation was Y=22 700X+2 510 (R2=0.9998).The RSD of IVM content in ivermectin microemulsion injection was 1.86%.The method was accurate and reliable,reproducible,easy to operate, which could be used in new type of ivermectin microemulsion injection.The method could be used as the basis of quality control and establishing a quality standard, and to provide basis for quality evaluation, also can provide reliable reference for safe veterinary clinical application in the future.关键词
伊维菌素微乳/伊维菌素/高效液相色谱法Key words
ivermectin microemulsion injection/ivermectin/high performance liquid chromatography分类
农业科技引用本文复制引用
王嗣涵,张继瑜,邢守叶,李冰,周绪正..伊维菌素微乳中伊维菌素的含量测定方法研究[J].中国畜牧兽医,2017,44(6):1861-1868,8.基金项目
国家现代农业肉牛牦牛产业技术体系建设专项资金(CARS-38) （CARS-38）
公益性行业(农业)科研专项经费(301303038-4) （农业）
