中国畜牧兽医2017，Vol.44Issue(6)：1884-1889,6.DOI:10.16431/j.cnki.1671-7236.2017.06.043
结核分枝杆菌耐药机制与检测技术研究进展
Research Progress on Resistance Mechanisms and Detecting Technology of Mycobacterium tuberculosis
摘要
Abstract
In recent years, the constantly emerging of drug resistant Mycobacterium tuberculosis brings unprecedented pressures to treat tuberculosis.In recent studies, it has found that not only bovine-type Mycobacterium tuberculosis can infect people, but human-type Mycobacterium tuberculosis can also infect cattle, which is also brings threats and challenges to the development of the cattle industry.And how to establish quick, handy, sensitive, highly specific and inexpensive resistance testing methods is the key to control the spread of the disease.In this review, it introduced not only the mechanism of action of isoniazid, rifampicin, streptomycin, ethambutol, pyrazinamide and so on, but also the traditional detection methods of drug resistance of mycobacteria (luciferase system, microscopic observation of drug susceptibility, proportion method, Bactec MGIT 960 System)and molecular detection methods (reverse hybriditation-based line probe assay (LiPA), Gene Xpert automatic detection system, PCR-single-strand conformation polymorphism analysis(PCR-SSCP),PCR-restriction fragment length polymorphism(PCR-RFLP), DNA sequencing, gene chip, RNA/RNA mismatch assay, which may be helpful to the further and clinical treatment and research for tuberculosis.关键词
结核分枝杆菌/耐药性检测/耐药机制Key words
Mycobacterium tuberculosis/detection of drug resistance/resistance mechanism分类
医药卫生引用本文复制引用
刘永安,李学瑞,刘永生..结核分枝杆菌耐药机制与检测技术研究进展[J].中国畜牧兽医,2017,44(6):1884-1889,6.基金项目
国家自然科学基金(31172335) （31172335）
