Abstract
ObjectiveTo establish the reference values of blood routine and serum iron in children aged 2~14 years in Tibet.MethodsChild Care Department, Maternity and Child Health Hospital of the People's Hospital of Tibet Autonomous Region was chosen in Tibet city of Lhasa (our hospital) as study site for preschool children.Three primary schools in Jiangzi County and Rerong Township, Lhunze County were chosen as study site for school-age children data.In this study, the parents of Tibetan children were Tibetans, and they lived for generations in Tibetan areas;the parents of Han children were Han nationality, and they had worked and lived in Tibet for a long time.The peripheral blood samples were collected from the children during the physical examination, and the blood routine and serum iron were tested in the clinical laboratory of our hospital.The test values were extracted from the relevant instruments and analyzed.LMS program software (version: 1.35) was used to fit the percentile curve of each index according to age.ResultsFrom Jan.1 to Dec.1 2016, 3 137 Tibetan and 744 Han healthy children aged 2 to 14 years were enrolled in this study.A total of 3 253 blood samples has been collected from preschool children and 628 blood samples from school aged children.There were no significant differences in gender between Tibetan and Han children at each age stage (P>0.05).Compared with the plain area, the reference ranges of RBC, Hb and PLT shifted right on the whole, both transferrin saturation(UIBC) and total iron binding capacity(TIBC) had a wider reference range;the reference range of WBC shifted left, and the reference ranges of neutrophil count (NEUT#), lymphocyte count (LYMPH#), monocyte count (MONO#) and the percentage of eosinophils (EO%) were wider, while that of basophil (BASO%) was narrower.As for absolute values, the reference range of NEUT# shifted left, and the reference ranges of LYMPH#, MONO#, eosinophil count (EO#) and BASO# were narrower.As age was increased, Hb, HCT, MPV, MCV, MCH, and RDW-SD showed an upward trend, PLT, LYMPH, BASO and LYMPH# showed a downward trend, while NEUT,NEUT# and PCT rose at first and then fell.There were significant differences in all 27 indexes except TIBC, MCH and PCT, between the Tibetan and Han children.WBC, RBC, Fe, Hb, HCT, PLT and MCV were higher in Han children than Tibetan children, MCHC, RDW-CV, PDW, UIBC, MPV, RDW-SD were just the opposite.Conclusion The reference values of blood routine and serum iron of Tibetan children changed with age mainly in the same way as Han children.The range of RBC, Hb and PLT, were wider and had a whole right shift, while the range of WBC was wider and had a whole left shift.The estimation of blood routine and serum iron in Tibetan children should be based on the local normal value.关键词
血常规/血清铁/参考值/儿童/高海拔/西藏Key words
Blood routine/Serum iron/Reference value/Children/High altitude/Tibet
