中国循证儿科杂志2017，Vol.12Issue(3)：180-184,5.DOI:10.3969/j.issn.1673-5501.2017.03.005
中国西部某二级医院呼吸道感染住院患儿鼻咽部肺炎链球菌中常见非PCV13血清型菌株的横断面调查
The non-PCV13 serotypes were frequent among Streptococcus pneumoniae isolated from nasopharyngeal samples of hospitalized children with respiratory infection in a grade two hospital in western China
摘要
Abstract
ObjectiveTo investigate the carriage rate, serotype distribution and antibiotic resistance profiles of Streptococcus neumoniae (S.pneumoniae) among hospitalized children with respiratory tract infections in grade two hospital in Zhongjiang County, Sichuan and to determine the epidemiological characters of these S.pneumoniae isolates.MethodsNasopharyngeal swabs of hospitalized children with respiratory tract infections aged from 1 month to 14 years were collected from January to December 2015 at the Zhongjiang County Hospital, and S.pneumoniae was cultured.The serotypes were detected by capsule swelling experiment to assess the coverage rate of 13-valent pneumococcal conjugate vaccine (PCV13).The sensitivity against 16 antimicrobial agents were detected by E-test or disk diffusion method for approximately half of S.pneumoniae isolates randomly selected based on the serotypes.ResultsThe nasopharyngeal carriage rate of S.pneumoniae was 18.4% (199/1082).The common serotypes were 19F (14.6%), 19A (10.6%), 34 (10.1%), 6A (9.0%), 23F (7.5%), 6B (7.5%) and 23A(7.0%).The coverage rate of PCV13 was 54.8%.The proportion of non-PCV13 types was up to 45.2%.All of the isolates were sensitive to penicillin using the parenteral breakpoints, but the sensitive rate decreased to 9.1% for the oral penicillin breakpoints.The sensitivity rate of amoxycillin/clavulanate was 97%.All of the isolates were sensitive to ceftriaxone, vancomycin, levofloxacin and linezolid.However, all of the isolates were resistant to erythromycin and azithromycin.ConclusionColonization of S.pneumoniae was frequent in the nasopharynx of children with respiratory tract infections in Zhongjiang.About half of the S.pneumoniae isolates were identified as the PCV13 types.Non-PCV13 serotypes were common in the county, in which type 34 and 23A were frequent, which should be monitored for long time and in wider range.The isolates showed a high resistance to oral penicillin and erythromycin, but sensitive to parenteral penicillin, ceftriaxone and vancomycin.关键词
肺炎链球菌/携带率/耐药性/血清型/儿童Key words
Streptococcus pneumoniae/Carriage rate/Antibiotic resistance/Serotype distribution/Children引用本文复制引用
文德年,刘莹,史伟,曾绍琦,曾海玲,唐萍,黄小刚,姚开虎..中国西部某二级医院呼吸道感染住院患儿鼻咽部肺炎链球菌中常见非PCV13血清型菌株的横断面调查[J].中国循证儿科杂志,2017,12(3):180-184,5.基金项目
1 国家自然科学基金:81371853 （）
2 国家科学技术部项目:2013BAI09B11 （）
3 北京市科委行业定额经费自主项目:2016-bjsekyjs-3 （）
