中国药科大学学报2017，Vol.48Issue(2)：208-213,6.DOI:10.11665/j.issn.1000-5048.20170212
17β-雌二醇与氟维司群抑制蛋白激酶C刺激下系膜细胞纤维化
17β-Estradiol and fulvestrant inhibit glomerular mesangial cell fibrogenesis downstream of protein kinase C activation
摘要
Abstract
To investigate the effect of estrogen receptor (ER) agonist 17β-estradiol and G protein-coupled estrogen receptor 1 (GPER) agonist fulvestrant on masangial cell fibrogenesis under protein kinase C (PKC),we quantified type Ⅳ collagen (COL4A1),fibronectin (FN1),connective tissue growth factor (CTGF) and transforming growth factor-β1 (TGFβ1) gene transcription and semi-quantified phosphorylation of Akt signal upon Phorbol 12-myristate 13-acetate stimulation (which increased COL4A1,FN1,CTGF and TGFβ1 gene transcription to 2.5-0.5,1.4 ±0.2,26 ± 11 and 1.9 ±0.3 times compared with baseline,P ＜0.05) when incubated with the two drugs.It was found that 17β-estradiol and fulvestrant down-regulated COL4A1,FN1,CTGF and TGFβ1 genes transcription (P ＜0.05) and Akt signaling under PKC activation via ER and GPER.ER and GPER agonists are beneficial in protecting the mesangial cells from fibrogenic stimuli by inhibiting PKC signaling and excessive extracellular matrix production.关键词
雌激素受体/17β-雌二醇/氟维司群/蛋白激酶C/肾小球系膜细胞Key words
estrogen receptors/17β-estradiol/fulvestrant/protein kinase C/glomerular mesangial cell分类
医药卫生引用本文复制引用
李一辰,史传林,丁选胜..17β-雌二醇与氟维司群抑制蛋白激酶C刺激下系膜细胞纤维化[J].中国药科大学学报,2017,48(2):208-213,6.基金项目
This study was supported by National Natural Science Foundation of China (No.81274158) （No.81274158）
the Fundamental Research Funds for the Central Universities (No.021414380053)国家自然科学基金资助项目(No.81274158) （No.021414380053）
中央高校基本科研业务费专项资金资助项目(No.021414380053) （No.021414380053）
