中国药科大学学报2017，Vol.48Issue(2)：214-219,6.DOI:10.11665/j.issn.1000-5048.20170213
葡萄球菌核酸酶的制备及性质研究
Studies on preparation and properties of Staphylococcus nuclease
摘要
Abstract
In order to study the relationship between Staphylococcal nuclease(SNase) and diabetes mellitus,genetic engineering bacteria E.coli BL21/pET28a-His-SNase was constructed,the expression of soluble extracellular protein SNase was induced and the a preliminary research was made on it.An expression vector pET28a-His-SNase plasmid containing the His-SNase gene was constructed and transformed into E.coli BL21 (DE3) competent cells.The protein was induced by lactose and purified by ultrasound destruction and Ni-affinity chromatography,respectively.It was then analyzed by SDS-PAGE and Western blot.The enzymatic properties for SNase has been preliminary studied as well.Results indicated that the purity of the correctly expressed fusion protein HisSNase was over 85％.SNase showed good activity within a wide range of pH and good heat resistance.This experiment might be a foundation work for the further study on the relationship between SNase and with diabetes.关键词
糖尿病/葡萄球菌核酸酶/组氨酸标签/分离纯化Key words
diabetes mellitus/Staphylococcal nuclease/His-tag/separation and purification分类
生物科学引用本文复制引用
洪流,郎君超,贺冬梅,刘坤锋,吴洁..葡萄球菌核酸酶的制备及性质研究[J].中国药科大学学报,2017,48(2):214-219,6.基金项目
This study was supported by National Natural Science Foundation of China (No.81673340,31270985) and the Natural Science Foundation of Jiangsu Province (No.BK20161462)国家自然科学基金资助项目(No.81673340,No.31270985) （No.81673340,31270985）
江苏省基础研究计划资助项目(No.BK20161462) （No.BK20161462）
