中国药房2017，Vol.28Issue(19)：2609-2612,4.DOI:10.6039/j.issn.1001-0408.2017.19.05
莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制研究
Study on the Preventive and Therapeutic Effect and Its Mechanism of Medicine Pair Decoction Liquid of Curcuma phaeocaulis-Sparganium stoloniferum on Uterine Myoma in Rats
摘要
Abstract
OBJECTIVE:To study the preventive and therapeutic effect and its mechanism of medicine pair decoction liquid of Curcuma phaeocaulis-Sparganium stoloniferum on rats with uterine myoma. METHODS:Rats were randomly divided into normal group,model group,positive group(mifepristone,2.25 mg/kg)and medicine pair decoction liquid group(C. phaeocaulis-S. stolon-iferum decoction liquid,6.0 g/kg),10 in each group. Except for the normal group,rats in other groups were injected Estradiol ben-zoate injection (0.5 mg/kg) intramuscularly every Monday,Wednesday and Friday,for 12 weeks. From the 13th week,rats in modeling group were added Progesterone injection(5 mg/kg)intramuscularly as well as relevant medicines intragastrically,once a day,until the 16th week. After administration,uterine coefficient of rats was detected. HE staining was used to observe the patho-logical changes of uterus and determine the thickness of smooth muscle;immunohistochemical staining was adopted to detect the transforming growth factor β3 (TGF-β3),matrix metalloproteinase 11 (MMP-11) protein expressions in uterus tissue of rats. RE-SULTS:Compared with normal group,uterine coefficient was increased in model group,pathological changes were obvious in uterus,thickness of smooth muscle was increased,TGF-β3 and MMP-11 protein expressions were enhanced(P<0.05 or P<0.01). Compared with model group,above-mentioned changes were improved significantly in positive group and medicine pair decoction liquid groups(P<0.05 or P<0.01). CONCLUSIONS:Medicine pair decoction liquid of C. phaeocaulis-S. stoloniferum shows cer-tain preventive and therapeutic effect on rats with uterine myoma. The mechanism may be associated with downregulating the TGF-β3,MMP-11 protein expressions in uterus tissue.关键词
莪术/三棱/药对/子宫肌瘤/转化生长因子β3/基质金属蛋白酶11/大鼠Key words
Curcuma phaeocaulis/Sparganium stoloniferum/Medicine pair/Uterine myoma/Transforming growth factor β3/Matrix metalloproteinase 11/Rats分类
医药卫生引用本文复制引用
李文静,李雪岩,崔涛,蔡德富,樊丽,张昊,刘雷,洪博..莪术-三棱药对合煎液对大鼠子宫肌瘤的防治作用及机制研究[J].中国药房,2017,28(19):2609-2612,4.基金项目
国家自然科学基金资助项目(No.81403173) （No.81403173）
