中国药房2017，Vol.28Issue(19)：2613-2616,4.DOI:10.6039/j.issn.1001-0408.2017.19.06
扶正解毒祛瘀方联合奥沙利铂对人结肠癌HT-29细胞增殖与凋亡的影响及机制研究
Study on the Effect and Its Mechanism of Fuzheng Jiedu Quyu Formula Combined with Oxaliplatin on Proliferation and Apoptosis of Human Colon Cancer HT-29 Cell
摘要
Abstract
OBJECTIVE:To study the effect and its mechanism of Fuzheng Jiedu Quyu formula(short for"Fuzheng formula") combined with oxaliplatin (L-OHP) on human colon cancer HT-29 cell proliferation and apoptosis. METHODS:HT-29 cells were divided into blank control group(without drugs),Fuzheng formula group(1000 mg/L),L-OHP group(31.25 mg/L)and combi-nation group (1000 mg/L Fuzheng formula+31.25 mg/L L-OHP). After cultured with corresponding drug for 48 h,MTT method was used to detect the cell proliferation;the changes of cellular morphology were observed by invert microscope;flow cytometry was used to detect the cell cycle and apoptosis rate. Proapoptotic gene Bax,apoptotic gene Bcl-2 mRNA expressions were deter-mined by real-time fluorescence quantitative polymerase chain reaction method;Bax,Bcl-2 protein expressions were assayed by Western blot. RESULTS:Compared with blank control group,cell proliferation was inhibited in L-OHP group and combination group;cell proportion was increased in S stage,G2/M stage and decreased in G0/G1 stage(P<0.05). Cell apoptosis rate in L-OHP group,Fuzheng formula group and combination group was increased;Bax mRNA and protein expression were up-regulated,Bcl-2 mRNA expression was downregulated(P<0.05);and combination group changed more obviously than the single drug groups(P<0.05). CONCLUSIONS:Fuzheng formula combined with L-OHP can inhibit HT-29 cell proliferation and promote its apoptosis, showing better effects than either of the two drugs alone. The mechanism may be associated with up-regulation of Bax gene and pro-tein expressions and down-regulation of Bcl-2 gene expressions in cells.关键词
扶正解毒祛瘀方/奥沙利铂/人结肠癌HT-29细胞/增殖/凋亡/体外Key words
Fuzheng Jiedu Quyu formula/Oxaliplatin/Human colon cancer HT-29 cell/Proliferation/Apoptosis/in vitro分类
医药卫生引用本文复制引用
赵秀梅,周冰,张桂贤,柴仲秋,刘晓芸,刘洪斌..扶正解毒祛瘀方联合奥沙利铂对人结肠癌HT-29细胞增殖与凋亡的影响及机制研究[J].中国药房,2017,28(19):2613-2616,4.基金项目
天津市中医药管理局中医中西医结合科研课题(No. 13164) （No. 13164）
天津市滨海新区塘沽科技发展专项资金项目(No.2013MS05-03) （No.2013MS05-03）
天津市滨海新区卫生局一般扶持项目(No.2014BWKY014) （No.2014BWKY014）
