中国药房2017，Vol.28Issue(19)：2624-2627,4.DOI:10.6039/j.issn.1001-0408.2017.19.09
舒肝宁注射液对大鼠酒精性肝纤维化的防治作用研究
Study on the Preventive and Therapeutic Effect of Shuganning Injection on Alcoholic Liver Fibrosis in Rats
摘要
Abstract
OBJECTIVE:To investigate the preventive and therapeutic effect of Shuganning injection on alcoholic liver fibrosis (ALF) in model rats,and provide experimental basis for its clinical application for alcoholic liver disease. METHODS:50 rats were enrolled and intraperitoneally given mixed liquid of 60% alcohol-corn oil-pyrazole to reduce ALF model. Another 10 rats were enrolled and intraperitoneally given normal saline,as normal control group. After 16 weeks,survived model rats(n=40)were ran-domly divided into model group,positive control group(Anluo huaxian pill 0.75 g/kg,ig),Shuganning injection high-dose,medi-um-dose,low-dose groups(4.8,2.4,1.2 mL/kg,ip),8 in each group. Normal control group and model group were intraperitone-ally injected equal volume of normal saline (5 mL/kg),administration groups were given relevant medicines,once a day,for 8 weeks;and modeling was contiuously conducted at the same time. After administration,body mass of rats was weighed,and the levels of liver function indexes [aspartate aminotransferase(AST),alanine aminotransferase(ALT)] and liver fibrosis indexes [hyal-uronic acid(HA),laminin(LN),type Ⅲ procollagen(PⅢNP),type Ⅳ collagen(Ⅳ-C)] in serum of rats were detected. Liver index of rats was determined and pathological changes of liver tissue were observed. RESULTS:Compared with normal control group,body mass of rats in model group was significantly decreased(P<0.05);liver index,and liver function index,liver fibro-sis index levels in serum were significantly increased (P<0.05 or P<0.01). Liver tissue showed steatosis,hepatocyte vacuoliza-tion,a large number of fibrous tissue deposition around portal areas and other pathological changes. Compared with model group,above-mentioned changes were improved significantly in administration groups (P<0.05 or P<0.01). CONCLUSIONS:Shugan-ning injection can obviously improve liver tissue damage of model rats with ALF,showing certain preventive and therapeutic effect on alcoholic liver disease.关键词
舒肝宁注射液/酒精性肝纤维化/大鼠Key words
Shuganning injection/Alcoholic liver fibrosis/Rats分类
医药卫生引用本文复制引用
潘婷,张金娟,熊英,李玲,雷珊,兰金芝..舒肝宁注射液对大鼠酒精性肝纤维化的防治作用研究[J].中国药房,2017,28(19):2624-2627,4.基金项目
贵州省科技计划课题(No.黔科合重大专项字〔2011〕6019号) （No.黔科合重大专项字〔2011〕6019号）
