中国药房2017，Vol.28Issue(19)：2677-2679,3.DOI:10.6039/j.issn.1001-0408.2017.19.24
正交试验优化独子藤果实中雷公藤红素的醇提工艺
Optimization of Methanol Extraction Technology for Tripterine in the Fruit of Celastrus monospermus by Orthogonal Design
摘要
Abstract
OBJECTIVE:To optimize the methanol extraction technology of tripterine in the fruit of Celastrus monospermus. METHODS:HPLC was adopted to determine the content of tripterine. Using extraction time,extraction times,solid-liquid ratio as investigation factors,extraction rate of tripterine as investigation index,orthogonal test was designed,and verification test was con-ducted. RESULTS:The optimal methanol extraction technology was as follow as 45% methanol extracting 2 h each time with sol-id-liquid ratio of 1:8 for 3 times. In the verification test,the average extraction rate of tripterine was 0.917 mg/g(RSD=2.85%, n=3). CONCLUSIONS:The optimized methanol extraction technology is stable and feasible with high extraction rate,and is suit-able for the extraction of tripterine in the fruit of C. monospermus.关键词
独子藤/雷公藤红素/单因素/正交试验/高效液相色谱法Key words
Celastrus monospermus/Tripterine/Single factor/Orthogonal test/HPLC分类
医药卫生引用本文复制引用
许启荣,周琼,张紫萍,陈纠..正交试验优化独子藤果实中雷公藤红素的醇提工艺[J].中国药房,2017,28(19):2677-2679,3.基金项目
广东省自筹经费类科技计划项目(No.粤科规财字〔2015〕110号) （No.粤科规财字〔2015〕110号）
