中国药理学通报2017，Vol.33Issue(8)：1068-1072,5.DOI:10.3969/j.issn.1001-1978.2017.08.007
腺病毒介导心脏特异性过表达RIP140对大鼠心脏功能及炎症通路的影响
Role of adenovirus-mediated cardiac-specificRIP140 overexpression in cardiac function and inflammation pathway
摘要
Abstract
Aim To explore the role of cardiac-specific overexpression of RIP140 in cardiac function and inflammation signaling pathway.Methods Direct intra-myocardial injection of adenovirus vector expressing RIP140 drove transgene expression in heart tissue.RIP140 overexpression was confirmed using immunofluorescence technique in heart.Cardiac function was assessed by echocardiographic and hemodynamic assessment.TNF-α,IL-2 and IL-1β inflammatory cytokines were detected by ELISA,and the protein levels of p65 and IκB-α were measured by Western blot.Results Adenovirus-mediated foreign gene of RIP140 was successfully transferred in cardiac tissue.RIP140 overexpression in heart induced ventricular dilation,decreased left ventricular ejection fraction and cardiac malfunction,as well as increased releases of TNF-α,IL-2 and IL-1β inflammatory cytokines,p65 protein translocation into the nucleus and IκB-α protein degradation in cytoplasm.Conclusion Adenovirus-mediated RIP140 overexpression in cardiac tissue impairs cardiac function,activates NF-κB/p65 inflammatory signaling pathway and induces the release of inflammatory cytokines.关键词
RIP140/炎症因子/NF-κB/心脏组织/腺病毒/能量代谢Key words
receptor interacting protein 140/inflammatory cytokines/nuclear factor κB/cardiac tissue/adenovirus/energy metabolism分类
医药卫生引用本文复制引用
陈艳芳,张銮坤,王若伦,刘培庆..腺病毒介导心脏特异性过表达RIP140对大鼠心脏功能及炎症通路的影响[J].中国药理学通报,2017,33(8):1068-1072,5.基金项目
国家自然科学基金资助项目(No 81402923) （No 81402923）
