中国药理学通报2017，Vol.33Issue(8)：1073-1079,7.DOI:10.3969/j.issn.1001-1978.2017.08.008
粗壮女贞总苷降脂作用及其基于AMPK通路的降脂作用机制研究
Hypolipidemic activity of total phenylpropanoid glycosides fromLigustrum robustum(Roxb.) Blume and its mechanisms on AMPK pathway
摘要
Abstract
Aim To evaluate the hypolipidemic effect of total phenylpropanoid glycoside extracted from Ligustrum robustum(Roxb.)Blume(CNTG)and its mecha-nisms.Methods The 60 hamsters were randomly divided into six groups,namely the control group,the model group,the positive control group(fenofibrate,150 mg·kg-1),the high(1 200 mg·kg-1),medium(600 mg·kg-1)and low(300 mg·kg-1)doses of CNTG groups.Only the control group was given control diet and other groups received high-fat diet.The changes of serum lipid were measured and analyzed in 1st week to ensure the successful establishment of the model.The drugs were administered daily for four weeks and the concentrations of lipids were determined in the 2nd week,3rd week and 4th week respectively.Quantitative real-time PCR and Western blot were used to assay the mRNA and protein expression of related signaling enzymes and proteins.Results Compared with the model group,the concentrations of serum TG,TC,LDL-C(P<0.05,P<0.01)and hepatic TG,TC(P<0.01)were effectively reduced in hamsters in CNTG-treated groups.Mechanism research found that CNTG increased the levels of phospho-AMPKα,LKB1 and phospho-LKB1 in liver(P<0.05).Conclusion CNTG prevents hyperlipidemia via activation of hepatic LKB1-AMPK pathway.关键词
粗壮女贞总苷/高脂血症/降脂/AMPK/LKB1/磷酸化Key words
total phenylpropanoid glycoside from Ligustrum robustum (Roxb.) Blume (CNTG)/hyperlipidemia/hypolipidemic/AMPK/LKB1/phosphorylation分类
医药卫生引用本文复制引用
孙乐,贺震旦,杨润梅,高南南,许利嘉,金文..粗壮女贞总苷降脂作用及其基于AMPK通路的降脂作用机制研究[J].中国药理学通报,2017,33(8):1073-1079,7.基金项目
协和青年科研基金资助项目(No 3332015142) （No 3332015142）
中国医学科学院医学与健康科技创新工程经费资助(No 2016-I2M-1-012) （No 2016-I2M-1-012）
