中国药理学通报2017，Vol.33Issue(8)：1079-1085,7.DOI:10.3969/j.issn.1001-1978.2017.08.009
蛇床子素上调miRNA-107减少APP595/596转基因神经元Aβ的生成
Osthole reduced Aβ synthesis by up-regulatingmiRNA-107 in neurons transfected with APP595/596 gene
摘要
Abstract
Aim To investigate the neuroprotective effects of osthole(Ost)on the primary cultured cortical neurons transfected with APP595/596 gene and its underlying mechanism.Methods Neonatal mouse cortical neurons were transfected with APP595/596 gene to establish AD cell models for the further study.Then,the cell viability was detected by CCK-8 assay,and the leakage of lactate dehydrogenase(LDH)was assayed by LDH kit to evaluate the injury degree.Transferase-mediated nick end labeling(TUNEL)was used to evaluate the cell apoptosis.The expression of β-amyloid peptide(Aβ)and β-site APP cleaving enzyme 1(BACE1)was measured by immunofluorescence,while the miRNA-107 was measured by RT-PCR.Results Compared to model group,Ost could significantly improve the neurons viability,decrease the LDH release and prevent the apoptosis.Ost also inhibited the expression of Aβ and BACE1 at protein level,while enhanced the expression of miRNA-107 at gene level.Conclusion Ost plays a neuroprotective role in neurons transfected with APP595/596 gene in part through up-regulating miRNA-107.关键词
独活/蛇床子素/阿尔茨海默病/神经保护/β-淀粉样蛋白/miRNA-107Key words
Angelicae Pubescentis Radix/osthole/Alzheimer's disease/neuroprotection/beta-amyloid peptide/miRNA-107分类
医药卫生引用本文复制引用
肖洪贺,教亚男,闫宇辉,李红艳,杨静娴..蛇床子素上调miRNA-107减少APP595/596转基因神经元Aβ的生成[J].中国药理学通报,2017,33(8):1079-1085,7.基金项目
国家自然科学基金资助项目(No81173580) （No81173580）
辽宁省自然科学基金项目(No201102144) （No201102144）
沈阳市科技专项资金(NoF11-264-1-42) （NoF11-264-1-42）
辽宁省高等学校优秀人才支持计划(5组191号) （5组191号）
