中国药理学通报2017，Vol.33Issue(8)：1086-1091,6.DOI:10.3969/j.issn.1001-1978.2017.08.010
姜黄素对鼻咽癌抗拒株的放射增敏作用及其机制研究
Effect of curcumin on radiosensitization of radioresistantnasopharyngeal carcinoma cell line CNE-2R and its mechanism
摘要
Abstract
Aim To investigate the effect of curcumin on radiosensitivity of radioresistant nasopharyngeal carcinoma cell line CNE-2R and its mechanism.Methods The concentration of curcumin was screened by MTT assay.Dose-survival curves were obtained according to the colony forming test for L-Q matching and multitarget-single hitting matching,while SF2 and the correlation parameters of radiation biology were calculated.The changes of cell cycle in CNE-2R cells caused by curcumin were also tested by flow cytometry(FCM).The differential expression of genes related to cell cycle and DNA damage repair were detected by RT-qPCR.Results CNE-2R cells could not be inhibited by 10 μmol·L-1 curcumin.Dealt with 10 μmol·L-1 curcumin for 24 h,the value of α/β increased to 1 596 from 6.56;the value of SF2 decreased to 0.361 Gy from 1.93 Gy;the value of N decreased to 1.06 from 1.60;the value of D0 decreased to 2.12 from 3.27;the value of Dq decreased to 0.12 from 1.53.FCM showed that the cells in G2 phase had a significant increase and the cells in S phase had a significant decrease after dealt with 10 μmol·L-1 curcumin for 24 h.The expression of CDK4 was significantly up-regulated and GADD45g,BRCA1 were significantly down-regulated.Conclusion Curcumin radiosensitizes nasopharyngeal carcinoma cell line CNE-2R by changing cell cycle and affecting DNA damage repair through regulating the expression of CDK4,GADD45 g and BRCA1.关键词
姜黄素/鼻咽癌/放射增敏/克隆形成/细胞周期/RT-qPCRKey words
curcumin/nasopharyngeal carcinoma/radiosensitization/colony formation assay/cell cycle/RT-qPCR分类
医药卫生引用本文复制引用
朱道琦,莫志贤,范钦,黄沐,刘钊汝,李爱武,邵萌,刘远亮,房淼,杨家彬,吕英..姜黄素对鼻咽癌抗拒株的放射增敏作用及其机制研究[J].中国药理学通报,2017,33(8):1086-1091,6.基金项目
国家自然科学基金资助项目(No 81173616,81673718,81229003,81673628) （No 81173616,81673718,81229003,81673628）
广东省自然科学基金资助项目(No 2016A030313833) （No 2016A030313833）
广东省科技计划资助项目(No 2013A032500003,2016A020226034) （No 2013A032500003,2016A020226034）
广州市重大科技资助项目(No 201300000050) （No 201300000050）
广州市白云区科技资助项目(No 2016-KJ-001) （No 2016-KJ-001）
